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Open data
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Basic information
Entry | Database: PDB / ID: 6r7s | ||||||
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Title | Human Serum Albumin, complexed with Sulfasalazine | ||||||
![]() | Serum albumin![]() | ||||||
![]() | LIPID BINDING PROTEIN / ![]() | ||||||
Function / homology | ![]() cellular response to calcium ion starvation / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Schreuder, H.A. / Liesum, A. | ||||||
![]() | ![]() Title: Resolving Binding Events on the Multifunctional Human Serum Albumin. Authors: Wenskowsky, L. / Wagner, M. / Reusch, J. / Schreuder, H. / Matter, H. / Opatz, T. / Petry, S.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 132.9 KB | Display | ![]() |
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PDB format | ![]() | 106.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4slyS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 66571.219 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() | ||||||
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#2: Chemical | ![]() #3: Chemical | ChemComp-SO4 / | ![]() #4: Chemical | ![]() #5: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.31 Å3/Da / Density % sol: 46.82 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: HSA was dissolved in 50 mM potassium phosphate, 150 mM sodium chloride (pH 7.5) and concentrated to 2 mM (140 mg/mL). The HSA solution was incubated with a six fold excess of sulfasalazine ...Details: HSA was dissolved in 50 mM potassium phosphate, 150 mM sodium chloride (pH 7.5) and concentrated to 2 mM (140 mg/mL). The HSA solution was incubated with a six fold excess of sulfasalazine at 4-5 degrees C for 4 hours.The crystal was grown by the hanging drop vapor diffusion method using a reservoir solution containing buffer (2.5 mM potassium phosphate, 7.5 mM sodium chloride, pH 7.0), 0.3% glycerol and polyethylene glycol 3350 (~30%). For crystallization 1 uL protein solution was equilibrated against 1 uL of reservoir solution. |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M-F / Detector: PIXEL / Date: Jul 20, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.208→48.616 Å / Num. obs: 18420 / % possible obs: 62.1 % / Redundancy: 3.4 % / Biso Wilson estimate: 48.12 Å2 / Rmerge(I) obs: 0.066 / Rsym value: 0.066 / Net I/σ(I): 12.7 |
Reflection shell | Resolution: 2.208→2.473 Å / Redundancy: 3.5 % / Rmerge(I) obs: 0.729 / Mean I/σ(I) obs: 1.6 / Num. unique obs: 922 / Rpim(I) all: 0.453 / Rrim(I) all: 0.85 / Rsym value: 0.729 / % possible all: 10.8 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 4sly Resolution: 2.21→48.616 Å / Cor.coef. Fo:Fc: 0.944 / Cor.coef. Fo:Fc free: 0.894 / Cross valid method: THROUGHOUT / σ(F): 0 / SU Rfree Blow DPI: 0.369
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Displacement parameters | Biso mean: 53.55 Å2
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Refine analyze | Luzzati coordinate error obs: 0.28 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.21→48.616 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.21→2.38 Å / Total num. of bins used: 43
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