+Open data
-Basic information
Entry | Database: PDB / ID: 6r2x | ||||||
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Title | NMR structure of Chromogranin A (F39-D63) | ||||||
Components | Chromogranin-A | ||||||
Keywords | HORMONE / fragment of Chromogranin A / ligand of alpha V beta 6 / interactors | ||||||
Function / homology | Function and homology information protein localization to secretory granule / positive regulation of relaxation of cardiac muscle / adenylate cyclase-activating adrenergic receptor signaling pathway involved in cardiac muscle relaxation / negative regulation of catecholamine secretion / positive regulation of dense core granule biogenesis / organelle organization / mast cell activation / mast cell chemotaxis / chromaffin granule / regulation of the force of heart contraction ...protein localization to secretory granule / positive regulation of relaxation of cardiac muscle / adenylate cyclase-activating adrenergic receptor signaling pathway involved in cardiac muscle relaxation / negative regulation of catecholamine secretion / positive regulation of dense core granule biogenesis / organelle organization / mast cell activation / mast cell chemotaxis / chromaffin granule / regulation of the force of heart contraction / Antimicrobial peptides / mast cell degranulation / positive regulation of phospholipase C-activating G protein-coupled receptor signaling pathway / neuronal dense core vesicle / negative regulation of insulin secretion / defense response to fungus / adenylate cyclase-activating adrenergic receptor signaling pathway / transport vesicle / positive regulation of cardiac muscle contraction / secretory granule / regulation of blood pressure / killing of cells of another organism / defense response to Gram-negative bacterium / defense response to Gram-positive bacterium / defense response to bacterium / innate immune response / perinuclear region of cytoplasm / extracellular space / extracellular region Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Nardelli, F. / Quilici, G. / Ghitti, M. / Curnis, F. / Gori, A. / Berardi, A. / Corti, A. / Musco, G. | ||||||
Citation | Journal: Chem.Commun.(Camb.) / Year: 2019 Title: A stapled chromogranin A-derived peptide is a potent dual ligand for integrins alpha v beta 6 and alpha v beta 8. Authors: Nardelli, F. / Ghitti, M. / Quilici, G. / Gori, A. / Luo, Q. / Berardi, A. / Sacchi, A. / Monieri, M. / Bergamaschi, G. / Bermel, W. / Chen, F. / Corti, A. / Curnis, F. / Musco, G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6r2x.cif.gz | 142.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6r2x.ent.gz | 120.9 KB | Display | PDB format |
PDBx/mmJSON format | 6r2x.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/r2/6r2x ftp://data.pdbj.org/pub/pdb/validation_reports/r2/6r2x | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 3042.471 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CHGA / Production host: Escherichia coli (E. coli) / References: UniProt: P10645 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 148 mM / Label: conditions_1 / pH: 6.5 / Pressure: 1 bar / Temperature: 280 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance 600 / Manufacturer: Bruker / Model: Avance 600 / Field strength: 600 MHz / Details: triple-resonance TCI cryo-probe |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 30 / Conformers submitted total number: 15 |