+Open data
-Basic information
Entry | Database: PDB / ID: 2kgn | ||||||
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Title | Solution structure of Ste5PM24 in the zwitterionic DPC micelle | ||||||
Components | Protein STE5 | ||||||
Keywords | SIGNALING PROTEIN / Ste5 / MAPKKK / Ste11 SAM / Ste50 SAM / Cytoplasm / Pheromone response / Phosphoprotein | ||||||
Function / homology | Function and homology information mating projection tip membrane / pheromone-dependent signal transduction involved in conjugation with cellular fusion / invasive growth in response to glucose limitation / MAP-kinase scaffold activity / mating projection tip / negative regulation of MAPK cascade / phosphatidylinositol-4,5-bisphosphate binding / kinase binding / G-protein beta-subunit binding / positive regulation of protein phosphorylation ...mating projection tip membrane / pheromone-dependent signal transduction involved in conjugation with cellular fusion / invasive growth in response to glucose limitation / MAP-kinase scaffold activity / mating projection tip / negative regulation of MAPK cascade / phosphatidylinositol-4,5-bisphosphate binding / kinase binding / G-protein beta-subunit binding / positive regulation of protein phosphorylation / nucleus / plasma membrane / cytoplasm Similarity search - Function | ||||||
Method | SOLUTION NMR / DGSA-distance geometry simulated annealing | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Bhunia, A. / Bhattacharjya, S. | ||||||
Citation | Journal: To be Published Title: Solution structure of Ste5PM24 in the zwitterionic DPC micelle Authors: Bhunia, A. / Bhattacharjya, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2kgn.cif.gz | 195.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2kgn.ent.gz | 165.5 KB | Display | PDB format |
PDBx/mmJSON format | 2kgn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kg/2kgn ftp://data.pdbj.org/pub/pdb/validation_reports/kg/2kgn | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 2897.471 Da / Num. of mol.: 1 / Fragment: UNP residues 44-67 / Source method: obtained synthetically / Details: This peptide is prepared synthetically. / References: UniProt: P32917 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 0.5mM Ste5 in 125mM DPC, 90% H2O/10% D2O / Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 0.5 mM / Component: Ste5, DPC |
Sample conditions | pH: 4.5 / Temperature: 308 K |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600 MHz |
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-Processing
NMR software | Name: DYANA / Version: 1.5 / Developer: Guntert, Braun, Wuthrich / Classification: refinement |
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Refinement | Method: DGSA-distance geometry simulated annealing / Software ordinal: 1 |
NMR representative | Selection criteria: lowest energy |
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 / Representative conformer: 1 |