+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 6k59 | ||||||
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タイトル | Structure of Glargine insulin in 20% acetic acid-d4 (pH 1.9) | ||||||
要素 |
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キーワード | HORMONE (ホルモン) / diabetes (糖尿病) / insulin (インスリン) | ||||||
機能・相同性 | 機能・相同性情報 negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / regulation of cellular amino acid metabolic process / negative regulation of fatty acid metabolic process / negative regulation of feeding behavior / Signaling by Insulin receptor / nitric oxide-cGMP-mediated signaling / IRS activation / Insulin processing / regulation of protein secretion ...negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / regulation of cellular amino acid metabolic process / negative regulation of fatty acid metabolic process / negative regulation of feeding behavior / Signaling by Insulin receptor / nitric oxide-cGMP-mediated signaling / IRS activation / Insulin processing / regulation of protein secretion / positive regulation of peptide hormone secretion / positive regulation of respiratory burst / Regulation of gene expression in beta cells / negative regulation of acute inflammatory response / alpha-beta T cell activation / negative regulation of respiratory burst involved in inflammatory response / positive regulation of dendritic spine maintenance / positive regulation of glycogen biosynthetic process / Synthesis, secretion, and deacylation of Ghrelin / negative regulation of protein secretion / positive regulation of nitric oxide mediated signal transduction / fatty acid homeostasis / regulation of protein localization to plasma membrane / Signal attenuation / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / negative regulation of lipid catabolic process / negative regulation of gluconeogenesis / COPI-mediated anterograde transport / positive regulation of lipid biosynthetic process / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / negative regulation of reactive oxygen species biosynthetic process / positive regulation of insulin receptor signaling pathway / 小胞 / positive regulation of protein autophosphorylation / Insulin receptor recycling / insulin-like growth factor receptor binding / neuron projection maintenance / NPAS4 regulates expression of target genes / positive regulation of protein metabolic process / positive regulation of brown fat cell differentiation / endoplasmic reticulum-Golgi intermediate compartment membrane / positive regulation of glycolytic process / activation of protein kinase B activity / positive regulation of mitotic nuclear division / Insulin receptor signalling cascade / positive regulation of cytokine production / positive regulation of long-term synaptic potentiation / Regulation of insulin secretion / acute-phase response / endosome lumen / positive regulation of protein secretion / positive regulation of glucose import / positive regulation of nitric-oxide synthase activity / positive regulation of cell differentiation / negative regulation of proteolysis / regulation of transmembrane transporter activity / wound healing / regulation of synaptic plasticity / insulin receptor binding / negative regulation of protein catabolic process / hormone activity / 認識 / positive regulation of neuron projection development / Golgi lumen / positive regulation of protein localization to nucleus / vasodilation / glucose metabolic process / regulation of protein localization / insulin receptor signaling pathway / cell-cell signaling / glucose homeostasis / positive regulation of NF-kappaB transcription factor activity / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / positive regulation of cell growth / secretory granule lumen / protease binding / positive regulation of MAPK cascade / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of cell migration / G protein-coupled receptor signaling pathway / Amyloid fiber formation / 小胞体 / ゴルジ体 / negative regulation of gene expression / positive regulation of cell population proliferation / regulation of DNA-templated transcription / positive regulation of gene expression / extracellular space / extracellular region / identical protein binding 類似検索 - 分子機能 | ||||||
生物種 | synthetic construct (人工物) | ||||||
手法 | 溶液NMR / simulated annealing | ||||||
データ登録者 | Ratha, B.N. / Kar, R.K. / Bhunia, A. | ||||||
資金援助 | インド, 1件
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引用 | ジャーナル: J.Phys.Chem.B / 年: 2020 タイトル: Molecular Details of a Salt Bridge and Its Role in Insulin Fibrillation by NMR and Raman Spectroscopic Analysis. 著者: Ratha, B.N. / Kar, R.K. / Bednarikova, Z. / Gazova, Z. / Kotler, S.A. / Raha, S. / De, S. / Maiti, N.C. / Bhunia, A. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 6k59.cif.gz | 165.1 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb6k59.ent.gz | 142.6 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 6k59.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/k5/6k59 ftp://data.pdbj.org/pub/pdb/validation_reports/k5/6k59 | HTTPS FTP |
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-関連構造データ
類似構造データ | |
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その他のデータベース |
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-リンク
-集合体
登録構造単位 |
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NMR アンサンブル |
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-要素
#1: タンパク質・ペプチド | 分子量: 2326.647 Da / 分子数: 1 / 由来タイプ: 合成 / 由来: (合成) synthetic construct (人工物) / 参照: UniProt: P01308*PLUS |
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#2: タンパク質・ペプチド | 分子量: 3748.340 Da / 分子数: 1 / 由来タイプ: 合成 / 由来: (合成) synthetic construct (人工物) / 参照: UniProt: P01308*PLUS |
-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||
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NMR実験 |
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-試料調製
詳細 | タイプ: solution 内容: 350 uM UNLABELLED PEPTIDE CHAIN A, 350 uM UNLABELLED PEPTIDE CHAIN B, 20% acetic acid/d4 詳細: 2mg/ml Zn free glargine insulin in 20% acetic acid/d4 at pH 1.9 Label: GI / 溶媒系: 20% acetic acid/d4 | ||||||||||||
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試料 |
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試料状態 | 詳細: 2mg/ml Zn free glargine insulin in 20% acetic acid/d4 at pH 1.9 イオン強度: 0 mM / Label: GI_20% / pH: 1.9 pD / 圧: 1 atm / 温度: 298 K |
-NMR測定
NMRスペクトロメーター | タイプ: Bruker AVANCE III / 製造業者: Bruker / モデル: AVANCE III / 磁場強度: 600 MHz / 詳細: IIT KGP |
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-解析
NMR software |
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精密化 | 手法: simulated annealing / ソフトェア番号: 5 | ||||||||||||||||||||||||
代表構造 | 選択基準: lowest energy | ||||||||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 50 / 登録したコンフォーマーの数: 10 |