PDB-5vl9: Crystal structure of EilR in complex with eilO DNA element

ID or keywords:

Entry

Database: PDB / ID: 5vl9

Title

Crystal structure of EilR in complex with eilO DNA element

Components

DNA (5'-D(*GP*AP*AP*AP*GP*TP*TP*GP*GP*AP*CP*AP*TP*A)-3')
DNA (5'-D(*TP*AP*TP*GP*TP*CP*CP*AP*AP*CP*TP*TP*TP*C)-3')
Regulatory protein TetR

Keywords

TRANSCRIPTION/DNA /

transcription factor /

protein-DNA complex / TRANSCRIPTION-DNA complex

Function / homology

Function and homology information

regulation of biosynthetic process / regulation of primary metabolic process /

Biological species

Enterobacter lignolyticus (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

SAD / Resolution: 2.16 Å

Authors

Pereira, J.H. / Ruegg, T.L. / Chen, J. / Novichkov, P. / DeGiovani, A. / Tomaleri, G.P. / Singer, S. / Simmons, B. / Thelen, M. / Adams, P.D.

Funding support

United States, 1items

Citation

Journal: Nat Commun / Year: 2018
Title: Jungle Express is a versatile repressor system for tight transcriptional control.
Authors: Ruegg, T.L. / Pereira, J.H. / Chen, J.C. / DeGiovanni, A. / Novichkov, P. / Mutalik, V.K. / Tomaleri, G.P. / Singer, S.W. / Hillson, N.J. / Simmons, B.A. / Adams, P.D. / Thelen, M.P.

History

Deposition	Apr 25, 2017	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Jun 27, 2018	Provider: repository / Type: Initial release
Revision 1.1	Nov 28, 2018	Group: Data collection / Database references / Category: citation / citation_author Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2	Feb 26, 2020	Group: Derived calculations Category: pdbx_struct_assembly / pdbx_struct_assembly_gen / pdbx_struct_assembly_prop Item: _pdbx_struct_assembly.details / _pdbx_struct_assembly.method_details ..._pdbx_struct_assembly.details / _pdbx_struct_assembly.method_details / _pdbx_struct_assembly.oligomeric_count / _pdbx_struct_assembly.oligomeric_details / _pdbx_struct_assembly_gen.asym_id_list
Revision 1.3	Mar 16, 2022	Group: Author supporting evidence / Database references / Category: database_2 / pdbx_audit_support Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_audit_support.funding_organization

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	5vl9.cif.gz	526.8 KB	Display	PDBx/mmCIF format
PDB format	pdb5vl9.ent.gz	458.8 KB	Display	PDB format
PDBx/mmJSON format	5vl9.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/vl/5vl9 ftp://data.pdbj.org/pub/pdb/validation_reports/vl/5vl9	HTTPS FTP

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Related structure data

Related structure data	5vlgC 5vlmC C: citing same article (ref.)
Similar structure data	2o3o-1 3lif-d 2o3o-6 2o3o-5 5vrf-d 6qyc-1 4xhq-d 2o3o-2 Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
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PDB pages

PDBj /

wwPDB /

NCBI

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Deposited unit

A: Regulatory protein TetR

B: Regulatory protein TetR

C: Regulatory protein TetR

D: Regulatory protein TetR

E: DNA (5'-D(*TP*AP*TP*GP*TP*CP*CP*AP*AP*CP*TP*TP*TP*C)-3')

F: DNA (5'-D(*GP*AP*AP*AP*GP*TP*TP*GP*GP*AP*CP*AP*TP*A)-3')

G: DNA (5'-D(*TP*AP*TP*GP*TP*CP*CP*AP*AP*CP*TP*TP*TP*C)-3')

H: DNA (5'-D(*GP*AP*AP*AP*GP*TP*TP*GP*GP*AP*CP*AP*TP*A)-3')

hetero molecules

107 kDa, 8 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: Regulatory protein TetR

B: Regulatory protein TetR

E: DNA (5'-D(*TP*AP*TP*GP*TP*CP*CP*AP*AP*CP*TP*TP*TP*C)-3')

F: DNA (5'-D(*GP*AP*AP*AP*GP*TP*TP*GP*GP*AP*CP*AP*TP*A)-3')

G: DNA (5'-D(*TP*AP*TP*GP*TP*CP*CP*AP*AP*CP*TP*TP*TP*C)-3')

H: DNA (5'-D(*GP*AP*AP*AP*GP*TP*TP*GP*GP*AP*CP*AP*TP*A)-3')

hetero molecules

defined by author
Evidence: native gel electrophoresis
61.9 kDa, 6 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

C: Regulatory protein TetR

D: Regulatory protein TetR

E: DNA (5'-D(*TP*AP*TP*GP*TP*CP*CP*AP*AP*CP*TP*TP*TP*C)-3')

F: DNA (5'-D(*GP*AP*AP*AP*GP*TP*TP*GP*GP*AP*CP*AP*TP*A)-3')

G: DNA (5'-D(*TP*AP*TP*GP*TP*CP*CP*AP*AP*CP*TP*TP*TP*C)-3')

H: DNA (5'-D(*GP*AP*AP*AP*GP*TP*TP*GP*GP*AP*CP*AP*TP*A)-3')

hetero molecules

defined by author
61.8 kDa, 6 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	54.790, 74.060, 81.360
Angle α, β, γ (deg.)	116.80, 105.19, 90.78
Int Tables number	1
Space group name H-M	P1

#1: Protein	Regulatory protein TetR Mass: 22084.943 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Enterobacter lignolyticus (bacteria) / Gene: Entcl_2353 / Production host: Escherichia coli (E. coli) / References: UniProt: E3G817
#2: DNA chain	DNA (5'-D(TPAPTPGPTPCPCPAPAPCPTPTPTPC)-3') Mass: 4205.752 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Enterobacter lignolyticus (bacteria)
#3: DNA chain	DNA (5'-D(GPAPAPAPGPTPTPGPGPAPCPAPTPA)-3') Mass: 4352.866 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Enterobacter lignolyticus (bacteria)
#4: Chemical	ChemComp-HEZ / HEXANE-1,6-DIOL / 1,6-Hexanediol Mass: 118.174 Da / Num. of mol.: 9 / Source method: isolated from a natural source / Formula: C₆H₁₄O₂
#5: Water	ChemComp-HOH / water / Water Mass: 18.015 Da / Num. of mol.: 266 / Source method: isolated from a natural source / Formula: H₂O

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.69 Å³/Da / Density % sol: 54.36 %
Crystal grow	Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 0.1 M Lithium sulphate, 0.1 M Magnesium chloride, 0.1 M Hepes pH 7.5, 20 %PEG 3,350 and 10 % Hexanediol

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Data collection

Diffraction	Mean temperature: 100 K
Diffraction source	Source: SYNCHROTRON / Site: ALS / Beamline: 8.2.2 / Wavelength: 0.97965 Å
Detector	Type: ADSC QUANTUM 315r / Detector: AREA DETECTOR / Date: Oct 14, 2014
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.97965 Å / Relative weight: 1
Reflection	Resolution: 2.16→65.29 Å / Num. obs: 55872 / % possible obs: 95.7 % / Redundancy: 3.2 % / CC_1/2: 0.995 / Net I/σ(I): 8.5

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Processing

Software

Name	Version	Classification
PHENIX	(dev_2650: ???)	refinement
xia2		data reduction
xia2		data scaling
AutoSol		phasing

Refinement

Method to determine structure:

SAD / Resolution: 2.16→65.288 Å / SU ML: 0.3 / Cross valid method: FREE R-VALUE / σ(F): 1.96 / Phase error: 27.22 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection
R_free	0.2288	2854	5.11 %
R_work	0.194	-	-
obs	0.1958	55872	95.63 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Refinement step

Cycle: LAST / Resolution: 2.16→65.288 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	6020	1136	72	266	7494

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.002	7465
X-RAY DIFFRACTION	f_angle_d	0.467	10289
X-RAY DIFFRACTION	f_dihedral_angle_d	14.151	4246
X-RAY DIFFRACTION	f_chiral_restr	0.033	1136
X-RAY DIFFRACTION	f_plane_restr	0.001	1133

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.16-2.1973	0.3659	112	0.3092	2724	X-RAY DIFFRACTION	96
2.1973-2.2372	0.3453	149	0.3009	2621	X-RAY DIFFRACTION	96
2.2372-2.2802	0.2882	145	0.2852	2673	X-RAY DIFFRACTION	96
2.2802-2.3268	0.3312	118	0.269	2673	X-RAY DIFFRACTION	96
2.3268-2.3774	0.3247	120	0.2573	2704	X-RAY DIFFRACTION	96
2.3774-2.4327	0.3311	152	0.2568	2596	X-RAY DIFFRACTION	95
2.4327-2.4935	0.3441	156	0.2462	2682	X-RAY DIFFRACTION	96
2.4935-2.5609	0.2589	155	0.2404	2659	X-RAY DIFFRACTION	96
2.5609-2.6363	0.2732	147	0.2332	2662	X-RAY DIFFRACTION	96
2.6363-2.7214	0.2578	148	0.232	2668	X-RAY DIFFRACTION	97
2.7214-2.8187	0.2697	156	0.2259	2650	X-RAY DIFFRACTION	96
2.8187-2.9315	0.2253	144	0.2225	2664	X-RAY DIFFRACTION	96
2.9315-3.0649	0.2494	140	0.2165	2695	X-RAY DIFFRACTION	97
3.0649-3.2265	0.2417	143	0.2092	2662	X-RAY DIFFRACTION	96
3.2265-3.4287	0.2303	110	0.1896	2685	X-RAY DIFFRACTION	96
3.4287-3.6934	0.1882	170	0.1677	2642	X-RAY DIFFRACTION	96
3.6934-4.065	0.2044	192	0.1611	2582	X-RAY DIFFRACTION	95
4.065-4.6531	0.1964	151	0.1463	2571	X-RAY DIFFRACTION	94
4.6531-5.8618	0.1883	134	0.167	2565	X-RAY DIFFRACTION	92
5.8618-65.3181	0.185	112	0.1534	2640	X-RAY DIFFRACTION	94

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	6.3864	-4.2536	4.7845	5.8047	-5.2485	9.1713	-0.1895	-0.432	0.5483	-0.3693	0.235	-0.1263	-0.4086	-0.602	0.0482	0.3004	0.0208	0.0373	0.3563	0.055	0.3609	-12.099	-66.6289	-0.3522
2	3.6319	0.4649	2.2312	1.8645	-0.2223	2.5063	0.0718	-0.0722	0.1716	-0.0657	-0.068	-0.0993	-0.0305	-0.2431	0.0243	0.3199	0.0273	0.0817	0.3505	0.0397	0.3119	-5.4225	-69.662	-0.2027
3	9.2415	0.7814	-3.1908	6.011	4.8048	9.0835	-0.2793	-0.6092	-0.2932	0.2054	0.1385	-0.0209	-0.1126	-0.1345	0.2979	0.4516	0.135	0.0557	0.451	0.0187	0.294	4.4516	-65.961	25.2032
4	2.8581	-0.6647	1.725	4.6503	1.7596	4.9193	-0.0922	-0.1373	0.2262	0.2212	0.2551	-0.0195	-0.2985	-0.3215	-0.1205	0.2396	0.039	0.0026	0.2579	0.0046	0.3289	7.4061	-68.3259	9.5138
5	3.3185	-2.118	-0.1084	5.4582	0.9367	3.9375	-0.2053	-0.3386	0.4513	0.2377	0.1318	-0.4953	-0.4972	-0.0818	0.1018	0.3002	0.0453	-0.0587	0.2031	-0.0735	0.3701	16.6881	-67.3504	18.764
6	5.2997	3.2987	2.0488	8.2995	2.0235	6.6273	-0.0575	-0.3338	-0.507	0.1694	0.2966	-0.5004	0.0619	-0.7057	-0.3365	0.3103	0.0415	0.0485	0.4101	0.0476	0.3981	15.8336	-75.4566	25.1894
7	6.2561	0.0315	-1.0934	8.6803	2.6098	5.2728	-0.0355	0.4325	-0.7137	-0.5264	0.0673	-0.4285	0.9034	0.127	0.053	0.6235	0.0905	-0.0121	0.5031	-0.0202	0.3179	28.36	-99.1744	-7.0737
8	7.8296	5.756	6.2429	7.4875	6.4182	7.5607	0.4486	0.12	-0.0306	0.4596	-0.3169	-0.0186	0.7923	0.1738	-0.137	0.4187	0.0688	0.0465	0.3487	0.0452	0.2565	30.1057	-90.632	4.238
9	6.1449	2.301	-0.1943	2.7969	1.3487	3.719	-0.5286	0.0238	-1.2037	-0.4495	-0.7231	-1.9448	0.3042	2.5526	1.0343	0.6565	0.0395	0.2343	0.6416	0.041	1.0906	43.7437	-77.3219	14.4958
10	4.1352	3.551	1.7567	4.1308	0.1772	7.3485	0.4331	0.2485	0.3213	0.1113	-0.1773	-0.4539	-0.2093	-0.0947	-0.1656	0.3161	0.0464	0.094	0.3088	0.0583	0.492	35.6307	-75.0716	14.381
11	9.4932	4.8457	-1.8317	3.4847	-0.5375	3.4481	0.4251	0.5503	0.8163	-0.1661	-0.1916	0.3921	-0.1652	-0.0085	-0.2638	0.3532	0.0327	0.0236	0.3462	0.0382	0.3962	23.2534	-82.8386	1.506
12	7.1964	5.5209	5.384	5.9269	4.8649	4.4416	-0.0066	0.1351	-0.1162	0.1697	0.1327	-0.055	0.3746	0.2109	-0.1943	0.3179	0.0314	0.0669	0.2752	0.0419	0.3257	26.4059	-86.618	15.8288
13	7.6936	-1.8688	5.8133	2.7359	-1.8725	8.2843	-0.2072	-0.3177	0.1095	0.2206	-0.0202	0.0402	-0.3363	-0.2763	0.27	0.3019	0.0428	0.0533	0.2373	-0.0194	0.2807	25.5402	-76.0503	20.2357
14	3.8089	-1.0659	3.7638	5.8441	-0.3761	3.7851	-0.6616	0.8337	1.2397	0.856	0.2914	-0.1562	-1.7621	-1.7142	0.7921	0.8713	0.1237	-0.0626	0.7768	0.1036	0.6413	19.7406	-64.5948	4.0911
15	4.9663	-4.0673	6.68	3.1602	-4.3996	2.0821	-0.3542	0.3008	0.7459	-0.1044	-0.4491	-0.7151	-0.4121	0.3185	0.583	0.3488	0.0039	0.0151	0.3775	0.0412	0.4682	29.0295	-67.8114	17.9712
16	6.0013	-2.9436	1.4153	3.4322	-4.0933	7.0812	-0.1759	-0.363	-0.1847	-0.2219	0.1959	-0.0942	0.6168	-0.2127	-0.1274	0.4748	-0.0325	-0.033	0.4262	0.0147	0.4205	13.1862	-100.2332	-31.4194
17	1.3586	-0.3038	-0.8998	2.7467	-3.2533	7.643	0.084	-0.0448	0.0939	-0.2395	-0.0573	-0.0159	-0.0316	0.0283	0.0079	0.4217	0.0729	-0.0195	0.3193	-0.0226	0.3683	18.5067	-91.3527	-42.9216
18	6.2812	-1.0207	0.3048	9.6462	7.0575	8.4405	-0.2902	-0.0356	0.0014	-0.1987	-0.1627	0.8327	-0.5513	-0.4548	0.4586	0.5221	0.093	0.0214	0.3596	0.1143	0.4165	9.9218	-84.5373	-40.662
19	4.8278	-0.8928	-1.5834	2.988	-0.6256	3.2499	0.3576	0.2202	0.4016	-0.1634	-0.2363	-0.3785	0.1753	0.2172	-0.1348	0.3925	0.0699	-0.0072	0.3032	0.0356	0.4594	20.3244	-77.1153	-49.3988
20	6.2357	0.0599	4.432	4.0325	1.7187	8.0064	0.7596	0.5452	-0.312	-0.7876	-0.3009	-0.0782	0.2651	-0.0138	-0.5064	0.6932	0.1849	-0.0239	0.3435	0.026	0.4232	15.9644	-74.4891	-60.4105
21	9.1985	-4.8474	0.0949	6.1401	-1.4326	6.4249	0.3128	0.4876	0.9185	0.9214	-0.3317	-0.051	-0.2595	-0.5511	-0.1207	0.7072	0.066	0.0809	0.453	-0.033	0.3859	-6.0837	-52.0187	-28.8191
22	8.1138	-3.4491	-0.6192	5.2422	4.0715	3.828	0.2116	-0.5165	0.0447	1.0762	-0.229	-0.399	0.2957	-0.1115	0.2816	0.7341	0.0164	0.0311	0.5999	0.0616	0.3435	-3.3193	-60.8828	-22.8429
23	2.7651	1.5598	3.1709	4.4953	4.9504	6.4328	-0.5637	-0.3522	0.497	0.5496	0.5208	0.9635	-0.5345	-0.4023	-0.0359	0.6223	0.1081	0.1155	0.6793	0.102	0.4121	-14.1085	-57.0404	-25.1868
24	9.3592	6.1297	-6.2939	6.338	-5.1293	4.3927	-0.0931	0.1748	0.0795	0.1903	0.1356	-0.1019	0.3056	-0.2853	-0.1121	0.4615	0.0415	-0.0338	0.3923	-0.0001	0.2828	0.6488	-56.352	-38.125
25	0.4251	-1.4441	-1.4979	6.1202	7.4832	9.7367	0.7228	-0.8131	1.4522	-0.5698	-0.6276	0.3798	-1.6865	-1.8039	-0.9085	0.8551	0.1937	0.1194	0.7398	-0.0902	0.9745	14.544	-46.7628	-50.1278
26	3.6682	1.2624	-3.205	4.5595	-2.3259	8.1434	0.335	0.1996	0.2716	0.7362	0.4824	0.1853	-0.2947	0.1693	-0.4634	0.3735	-0.0245	-0.0206	0.2876	0.0096	0.4416	16.2679	-53.962	-49.4125
27	7.8006	5.023	-0.9659	4.0683	-1.5039	6.8718	0.0231	-0.2218	-0.2429	1.2373	0.2563	-0.4339	-0.171	-0.0247	-0.1744	0.4757	0.1507	-0.0184	0.3994	0.0308	0.3488	7.2721	-64.3	-35.2696
28	3.9278	2.3416	-3.0408	3.7447	-3.5279	7.5122	0.0245	0.2393	-0.058	-0.1316	-0.0391	-0.0866	0.0398	-0.2074	-0.0267	0.2994	0.0523	0.0045	0.2782	0.0079	0.291	5.8754	-60.5324	-53.1323
29	6.2639	-4.4929	3.8353	6.556	-4.9166	7.3643	-0.2687	-0.217	0.2006	0.4928	0.0944	-0.1837	-0.7177	-0.0097	0.0544	0.3661	0.0338	0.0155	0.2643	-0.0198	0.3462	13.3345	-67.322	-48.5767
30	3.7705	-2.6032	2.889	8.4583	0.5175	3.301	-0.072	-0.2734	0.1733	0.6009	0.1531	-0.6185	0.0657	0.4045	-0.5414	0.3782	0.076	0.0368	0.463	0.0814	0.5962	23.1744	-63.888	-49.4483
31	0.5751	-0.0881	1.5111	5.9869	0.465	5.6225	0.0558	-0.2354	-0.2411	1.2219	-0.299	-0.1315	-0.0184	-0.4855	0.1434	0.4588	-0.0315	0.0803	0.663	0.1184	0.5112	12.1094	-104.9232	-16.2893
32	6.47	-5.4613	-0.6259	9.1449	3.697	4.9584	-0.8255	-0.0453	-0.0516	1.7184	0.1984	0.2445	0.0716	-0.253	0.4848	0.762	0.0589	0.0355	0.6013	0.1732	0.5443	13.596	-105.0652	-14.4862
33	7.5292	-3.9204	-0.7458	3.8865	-1.7826	5.0015	0.063	0.7268	-0.219	-0.551	-0.0715	0.4781	0.4567	-0.1856	-0.0412	0.5389	-0.0699	-0.0766	0.4052	0.0351	0.3856	-16.1413	-69.6791	-16.2853
34	4.3647	-2.7852	-2.9796	7.5953	0.0572	8.5704	-0.3565	0.4624	-0.0806	-0.1169	0.0257	0.1107	0.7	0.1941	0.385	0.4911	-0.0284	-0.0436	0.5431	0.107	0.3679	-15.8289	-68.2773	-18.0085

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 2 through 24 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 25 through 72 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 73 through 91 )
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 92 through 138 )
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 139 through 168 )
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 169 through 192 )
7	X-RAY DIFFRACTION	7	chain 'B' and (resid 5 through 51 )
8	X-RAY DIFFRACTION	8	chain 'B' and (resid 52 through 73 )
9	X-RAY DIFFRACTION	9	chain 'B' and (resid 74 through 78 )
10	X-RAY DIFFRACTION	10	chain 'B' and (resid 79 through 91 )
11	X-RAY DIFFRACTION	11	chain 'B' and (resid 92 through 111 )
12	X-RAY DIFFRACTION	12	chain 'B' and (resid 112 through 138 )
13	X-RAY DIFFRACTION	13	chain 'B' and (resid 139 through 166 )
14	X-RAY DIFFRACTION	14	chain 'B' and (resid 167 through 174 )
15	X-RAY DIFFRACTION	15	chain 'B' and (resid 175 through 192 )
16	X-RAY DIFFRACTION	16	chain 'C' and (resid 2 through 38 )
17	X-RAY DIFFRACTION	17	chain 'C' and (resid 39 through 91 )
18	X-RAY DIFFRACTION	18	chain 'C' and (resid 92 through 111 )
19	X-RAY DIFFRACTION	19	chain 'C' and (resid 112 through 174 )
20	X-RAY DIFFRACTION	20	chain 'C' and (resid 175 through 192 )
21	X-RAY DIFFRACTION	21	chain 'D' and (resid 4 through 24 )
22	X-RAY DIFFRACTION	22	chain 'D' and (resid 25 through 38 )
23	X-RAY DIFFRACTION	23	chain 'D' and (resid 39 through 51 )
24	X-RAY DIFFRACTION	24	chain 'D' and (resid 52 through 73 )
25	X-RAY DIFFRACTION	25	chain 'D' and (resid 74 through 78 )
26	X-RAY DIFFRACTION	26	chain 'D' and (resid 79 through 91 )
27	X-RAY DIFFRACTION	27	chain 'D' and (resid 92 through 111 )
28	X-RAY DIFFRACTION	28	chain 'D' and (resid 112 through 147 )
29	X-RAY DIFFRACTION	29	chain 'D' and (resid 148 through 168 )
30	X-RAY DIFFRACTION	30	chain 'D' and (resid 169 through 192 )
31	X-RAY DIFFRACTION	31	chain 'E' and (resid 1 through 14 )
32	X-RAY DIFFRACTION	32	chain 'F' and (resid 1 through 14 )
33	X-RAY DIFFRACTION	33	chain 'G' and (resid 1 through 14 )
34	X-RAY DIFFRACTION	34	chain 'H' and (resid 1 through 14 )

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