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Open data
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Basic information
Entry | Database: PDB / ID: 5mjw | ||||||
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Title | Structure of Psb29 at 1.55A | ||||||
![]() | Protein Thf1 | ||||||
![]() | ![]() ![]() | ||||||
Function / homology | Protein Thf1 / Thylakoid formation protein / ![]() ![]() | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Murray, J.W. / Kozlo, A. | ||||||
![]() | ![]() Title: Structure of Psb29/Thf1 and its association with the FtsH protease complex involved in photosystem II repair in cyanobacteria. Authors: Bec Kova, M. / Yu, J. / Krynicka, V. / Kozlo, A. / Shao, S. / Konik, P. / Komenda, J. / Murray, J.W. / Nixon, P.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 95.1 KB | Display | ![]() |
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PDB format | ![]() | 72.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5mjoSC ![]() 5mjrC ![]() 5mlfC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 27268.848 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: BP-1 / Gene: thf1, tlr1134 / Plasmid: pRSETa modified / Production host: ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.53 Å3/Da / Density % sol: 65.16 % |
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Crystal grow![]() | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7 / Details: 0.2M sodium malonate, pH 7, 20% w/v/ PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Feb 5, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.47→55.26 Å / Num. obs: 11662 / % possible obs: 99.64 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 6.34 % / Rmerge(I) obs: 0.062 / Net I/av σ(I): 16.5176 / Net I/σ(I): 6.2893 |
Reflection shell | Resolution: 2.47→2.53 Å / Redundancy: 6.45 % / Rmerge(I) obs: 0.83 / Mean I/σ(I) obs: 0.67 / % possible all: 98.77 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 5MJO Resolution: 2.47→55.26 Å / Cor.coef. Fo:Fc: 0.949 / Cor.coef. Fo:Fc free: 0.94 / SU B: 18.463 / SU ML: 0.186 / Cross valid method: THROUGHOUT / ESU R: 0.315 / ESU R Free: 0.237 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 75.277 Å2
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Refinement step | Cycle: 1 / Resolution: 2.47→55.26 Å
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Refine LS restraints |
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