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Yorodumi- PDB-5m4w: Structure of DNA AGCGA-quadruplex adopted by 15-mer oligonucleoti... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5m4w | |||||||||||||||||||||||||||||||
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Title | Structure of DNA AGCGA-quadruplex adopted by 15-mer oligonucleotide found in regulatory region of the PLEKHG3 human gene with G11 to I11 mutation, d(GCGAGGGAGCIAGGG),VK34_I11 | |||||||||||||||||||||||||||||||
Components | DNA (5'-D(*Keywords | DNA / AGCGA-quadruplex / AGCGA-quadruplex family / G-G N1-carboniyl symmetric base pair / PLEKHG3 gene / inosine | Function / homology | DNA / DNA (> 10) | Function and homology information Biological species | Homo sapiens (human) | Method | SOLUTION NMR / simulated annealing | Authors | Kocman, V. / Plavec, J. | Funding support | Slovenia, 2items |
Citation | Journal: Nat Commun / Year: 2017 | Title: Tetrahelical structural family adopted by AGCGA-rich regulatory DNA regions. Authors: Kocman, V. / Plavec, J. History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5m4w.cif.gz | 197.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5m4w.ent.gz | 158.4 KB | Display | PDB format |
PDBx/mmJSON format | 5m4w.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m4/5m4w ftp://data.pdbj.org/pub/pdb/validation_reports/m4/5m4w | HTTPS FTP |
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-Related structure data
Related structure data | 5m1lC 5m2lC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: DNA chain | Mass: 4750.071 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 100 mM / Label: VK34_I11_c1 / pH: 6 / Pressure: 1 atm / Temperature: 273 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 10 |