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Yorodumi- PDB-5jwo: Crystal structure of foldswitch-stabilized KaiB in complex with t... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5jwo | |||||||||
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Title | Crystal structure of foldswitch-stabilized KaiB in complex with the N-terminal CI domain of KaiC from Thermosynechococcus elongatus | |||||||||
Components |
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Keywords | Transcription Regulator / foldswitch | |||||||||
Function / homology | Function and homology information negative regulation of phosphorylation / protein serine/threonine/tyrosine kinase activity / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / regulation of circadian rhythm / circadian rhythm / kinase activity / protein autophosphorylation / non-specific serine/threonine protein kinase / protein serine kinase activity / protein serine/threonine kinase activity ...negative regulation of phosphorylation / protein serine/threonine/tyrosine kinase activity / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / regulation of circadian rhythm / circadian rhythm / kinase activity / protein autophosphorylation / non-specific serine/threonine protein kinase / protein serine kinase activity / protein serine/threonine kinase activity / regulation of DNA-templated transcription / magnesium ion binding / ATP hydrolysis activity / DNA binding / ATP binding / identical protein binding Similarity search - Function | |||||||||
Biological species | Thermosynechococcus elongatus (bacteria) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.8 Å | |||||||||
Authors | Tseng, R. / Goularte, N.F. / Chavan, A. / Luu, J. / Chang, Y. / Heilser, J. / Tripathi, S. / LiWang, A. / Partch, C.L. | |||||||||
Funding support | United States, 2items
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Citation | Journal: Science / Year: 2017 Title: Structural basis of the day-night transition in a bacterial circadian clock. Authors: Tseng, R. / Goularte, N.F. / Chavan, A. / Luu, J. / Cohen, S.E. / Chang, Y.G. / Heisler, J. / Li, S. / Michael, A.K. / Tripathi, S. / Golden, S.S. / LiWang, A. / Partch, C.L. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5jwo.cif.gz | 133.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5jwo.ent.gz | 104.2 KB | Display | PDB format |
PDBx/mmJSON format | 5jwo.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jw/5jwo ftp://data.pdbj.org/pub/pdb/validation_reports/jw/5jwo | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 27738.166 Da / Num. of mol.: 1 / Mutation: R41A, K173A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermosynechococcus elongatus (bacteria) Strain: BP-1 / Gene: kaiC, tlr0483 / Plasmid: pET-28b / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) References: UniProt: Q79V60, non-specific serine/threonine protein kinase |
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#2: Protein | Mass: 10941.011 Da / Num. of mol.: 1 / Mutation: Y8A, G89A, D91R, Y94A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermosynechococcus elongatus (strain BP-1) (bacteria) Strain: BP-1 / Gene: kaiB, tlr0482 / Plasmid: pET-28b / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: Q79V61 |
#3: Chemical | ChemComp-ADP / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.35 Å3/Da / Density % sol: 47.57 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop Details: 0.2M Potassium Acetate, 10% (w/v) PEG3350, and 2.5% glycerol |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 8.3.1 / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Sep 11, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→73.07 Å / Num. obs: 32745 / % possible obs: 97.8 % / Redundancy: 3.4 % / CC1/2: 0.987 / Rmerge(I) obs: 0.1 / Net I/σ(I): 6.5 |
Reflection shell | Resolution: 1.8→1.9 Å / Redundancy: 3.6 % / Rmerge(I) obs: 0.44 / Mean I/σ(I) obs: 3.1 / % possible all: 99.3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.8→33.973 Å / SU ML: 0.2 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 24.78
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→33.973 Å
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Refine LS restraints |
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LS refinement shell |
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