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Yorodumi- PDB-5jyu: NMR structure of pseudo receiver domain of CikA from Thermosynech... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5jyu | ||||||
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Title | NMR structure of pseudo receiver domain of CikA from Thermosynechococcus elongatus | ||||||
Components | Two-component sensor histidine kinase | ||||||
Keywords | Sigaling protein / Circadian Clock / Signaling protein | ||||||
Function / homology | Function and homology information histidine kinase / phosphorelay sensor kinase activity / rhythmic process / cell cycle / cell division / ATP binding Similarity search - Function | ||||||
Biological species | Thermosynechococcus elongatus (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Tseng, R.D. / LiWang, A.L. | ||||||
Funding support | United States, 1items
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Citation | Journal: Science / Year: 2017 Title: Structural basis of the day-night transition in a bacterial circadian clock. Authors: Tseng, R. / Goularte, N.F. / Chavan, A. / Luu, J. / Cohen, S.E. / Chang, Y.G. / Heisler, J. / Li, S. / Michael, A.K. / Tripathi, S. / Golden, S.S. / LiWang, A. / Partch, C.L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5jyu.cif.gz | 709 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5jyu.ent.gz | 594.9 KB | Display | PDB format |
PDBx/mmJSON format | 5jyu.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jy/5jyu ftp://data.pdbj.org/pub/pdb/validation_reports/jy/5jyu | HTTPS FTP |
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-Related structure data
Related structure data | 5jwoC 5jwqC 5jwrC 5jytC 5jyvC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 12899.736 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermosynechococcus elongatus (strain BP-1) (bacteria) Strain: BP-1 / Gene: tll0899 / Plasmid: pET28b Production host: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria) References: UniProt: Q8DKG0 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 0.1 mM / Label: condition_1 / pH: 7 / Pressure: 1 atm / Temperature: 25 C |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE III / Manufacturer: Bruker / Model: AVANCE III / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |