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Yorodumi- PDB-5j3h: Human insulin receptor domains L1-CR in complex with peptide S519... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5j3h | |||||||||||||||
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Title | Human insulin receptor domains L1-CR in complex with peptide S519C16 and 83-7 Fv | |||||||||||||||
Components |
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Keywords | HORMONE RECEPTOR/IMMUNE SYSTEM / Insulin receptor Insulin mimetic peptide Fv fragment / HORMONE RECEPTOR-IMMUNE SYSTEM complex | |||||||||||||||
Function / homology | Function and homology information regulation of female gonad development / positive regulation of meiotic cell cycle / positive regulation of developmental growth / insulin-like growth factor II binding / male sex determination / exocrine pancreas development / insulin receptor complex / insulin-like growth factor I binding / insulin receptor activity / positive regulation of protein-containing complex disassembly ...regulation of female gonad development / positive regulation of meiotic cell cycle / positive regulation of developmental growth / insulin-like growth factor II binding / male sex determination / exocrine pancreas development / insulin receptor complex / insulin-like growth factor I binding / insulin receptor activity / positive regulation of protein-containing complex disassembly / cargo receptor activity / dendritic spine maintenance / insulin binding / PTB domain binding / adrenal gland development / neuronal cell body membrane / Signaling by Insulin receptor / IRS activation / activation of protein kinase activity / amyloid-beta clearance / positive regulation of respiratory burst / regulation of embryonic development / positive regulation of receptor internalization / transport across blood-brain barrier / insulin receptor substrate binding / positive regulation of glycogen biosynthetic process / epidermis development / Signal attenuation / phosphatidylinositol 3-kinase binding / heart morphogenesis / dendrite membrane / Insulin receptor recycling / insulin-like growth factor receptor binding / neuron projection maintenance / positive regulation of glycolytic process / activation of protein kinase B activity / positive regulation of mitotic nuclear division / receptor-mediated endocytosis / Insulin receptor signalling cascade / learning / caveola / positive regulation of glucose import / positive regulation of MAP kinase activity / receptor internalization / receptor protein-tyrosine kinase / memory / cellular response to growth factor stimulus / peptidyl-tyrosine phosphorylation / cellular response to insulin stimulus / positive regulation of nitric oxide biosynthetic process / male gonad development / late endosome / insulin receptor signaling pathway / glucose homeostasis / amyloid-beta binding / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / protein tyrosine kinase activity / positive regulation of MAPK cascade / protein autophosphorylation / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / lysosome / receptor complex / endosome membrane / positive regulation of cell migration / symbiont entry into host cell / positive regulation of protein phosphorylation / G protein-coupled receptor signaling pathway / protein domain specific binding / external side of plasma membrane / axon / protein phosphorylation / positive regulation of cell population proliferation / protein-containing complex binding / regulation of DNA-templated transcription / GTP binding / positive regulation of DNA-templated transcription / extracellular exosome / ATP binding / membrane / identical protein binding / plasma membrane Similarity search - Function | |||||||||||||||
Biological species | Mus musculus (house mouse) Homo sapiens (human) Endothia gyrosa (fungus) | |||||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.27 Å | |||||||||||||||
Authors | Lawrence, M. / Menting, J. / Lawrence, C. | |||||||||||||||
Funding support | Australia, 4items
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Citation | Journal: J.Biol.Chem. / Year: 2016 Title: Insulin Mimetic Peptide Disrupts the Primary Binding Site of the Insulin Receptor. Authors: Lawrence, C.F. / Margetts, M.B. / Menting, J.G. / Smith, N.A. / Smith, B.J. / Ward, C.W. / Lawrence, M.C. | |||||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5j3h.cif.gz | 229.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5j3h.ent.gz | 184 KB | Display | PDB format |
PDBx/mmJSON format | 5j3h.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/j3/5j3h ftp://data.pdbj.org/pub/pdb/validation_reports/j3/5j3h | HTTPS FTP |
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-Related structure data
Related structure data | 4ogaS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Antibody , 2 types, 2 molecules CD
#2: Antibody | Mass: 13440.069 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Production host: Brevibacillus (bacteria) |
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#3: Antibody | Mass: 13152.705 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Production host: Brevibacillus (bacteria) |
-Protein/peptide / Protein , 2 types, 2 molecules BE
#1: Protein/peptide | Mass: 1950.047 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: 16 C-terminal residues of peptide S519: SER-LEU-ASP-GLU-SER-PHE-TYR-ASP-TRP-PHE-GLU-ARG-GLN-LEU Source: (synth.) Endothia gyrosa (fungus) |
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#4: Protein | Mass: 36231.762 Da / Num. of mol.: 1 / Fragment: UNP residues 28-337 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: INSR / Production host: Cricetulus griseus (Chinese hamster) / Strain (production host): Lec8 References: UniProt: P06213, receptor protein-tyrosine kinase |
-Sugars , 4 types, 5 molecules
#5: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta- ...2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source |
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#6: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source |
#7: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose- ...2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source |
#8: Sugar |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 5.2 Å3/Da / Density % sol: 76.33 % |
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Crystal grow | Temperature: 300 K / Method: vapor diffusion, sitting drop / Details: 1.75 M (NH4)2HPO4 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Australian Synchrotron / Beamline: MX2 / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jul 1, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3.27→34.79 Å / Num. obs: 21483 / % possible obs: 99.4 % / Redundancy: 6.3 % / Biso Wilson estimate: 85.67 Å2 / Net I/σ(I): 7.68 |
Reflection shell | Resolution: 3.27→3.39 Å / Redundancy: 6.38 % / Rmerge(I) obs: 2.23 / Mean I/σ(I) obs: 1 / % possible all: 98.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4OGA Resolution: 3.27→34.79 Å / Cor.coef. Fo:Fc: 0.9116 / Cor.coef. Fo:Fc free: 0.8871 / SU R Cruickshank DPI: 0.72 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.725 / SU Rfree Blow DPI: 0.357 / SU Rfree Cruickshank DPI: 0.361
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Displacement parameters | Biso mean: 122.88 Å2
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Refine analyze | Luzzati coordinate error obs: 0.899 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.27→34.79 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.27→3.43 Å / Total num. of bins used: 11
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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