+Open data
-Basic information
Entry | Database: PDB / ID: 5gha | ||||||
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Title | Sulfur Transferase TtuA in complex with Sulfur Carrier TtuB | ||||||
Components |
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Keywords | TRANSFERASE/TRANSPORT PROTEIN / sulfur transferase / TRANSFERASE / TRANSFERASE-TRANSPORT PROTEIN complex | ||||||
Function / homology | Function and homology information tRNA-5-methyluridine54 2-sulfurtransferase / tRNA thio-modification / tRNA wobble uridine modification / tRNA processing / 4 iron, 4 sulfur cluster binding / transferase activity / tRNA binding / nucleotide binding / ATP binding / metal ion binding Similarity search - Function | ||||||
Biological species | Thermus thermophilus HB27 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 2.502 Å | ||||||
Authors | Chen, M. / Narai, S. / Tanaka, Y. / Yao, M. | ||||||
Citation | Journal: Proc. Natl. Acad. Sci. U.S.A. / Year: 2017 Title: Biochemical and structural characterization of oxygen-sensitive 2-thiouridine synthesis catalyzed by an iron-sulfur protein TtuA Authors: Chen, M. / Asai, S. / Narai, S. / Nambu, S. / Omura, N. / Sakaguchi, Y. / Suzuki, T. / Ikeda-Saito, M. / Watanabe, K. / Yao, M. / Shigi, N. / Tanaka, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5gha.cif.gz | 300 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5gha.ent.gz | 243 KB | Display | PDB format |
PDBx/mmJSON format | 5gha.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gh/5gha ftp://data.pdbj.org/pub/pdb/validation_reports/gh/5gha | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
-Protein , 2 types, 8 molecules ADBCEHFG
#1: Protein | Mass: 36252.246 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermus thermophilus HB27 (bacteria) / Strain: HB27 / Gene: TT_C0106 / Production host: Escherichia coli (E. coli) / References: UniProt: Q72LF3 #2: Protein | Mass: 9536.782 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Details: G65C mutant / Source: (gene. exp.) Thermus thermophilus HB27 (bacteria) / Strain: HB27 / Gene: TT_C0105 / Production host: Escherichia coli (E. coli) / References: UniProt: Q72LF4 |
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-Non-polymers , 4 types, 98 molecules
#3: Chemical | ChemComp-ZN / #4: Chemical | #5: Chemical | ChemComp-EDO / #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.47 Å3/Da / Density % sol: 45.53 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion / Details: 0.1 M HEPES-NaOH, 0-20%(v/v) PEG6000 / PH range: 6.2-7.2 |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: Photon Factory / Beamline: AR-NE3A / Wavelength: 1.28251, 1.28311, 1.00000 | ||||||||||||
Detector | Type: ADSC QUANTUM 270 / Detector: CCD / Date: Dec 15, 2012 | ||||||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2.5→47.84 Å / Num. obs: 105373 / % possible obs: 94.9 % / Redundancy: 1.97 % / Net I/σ(I): 16.24 |
-Processing
Software |
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Refinement | Method to determine structure: MAD / Resolution: 2.502→47.84 Å / SU ML: 0.34 / Cross valid method: FREE R-VALUE / σ(F): 1.98 / Phase error: 27.05 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.502→47.84 Å
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Refine LS restraints |
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LS refinement shell |
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