+Open data
-Basic information
Entry | Database: PDB / ID: 5cvl | ||||||
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Title | WDR48 (UAF-1), residues 2-580 | ||||||
Components | WD repeat-containing protein 48 | ||||||
Keywords | PROTEIN BINDING / WDR48 / UAF1 / WD-repeat / USP / deubiquitinase / DUB | ||||||
Function / homology | Function and homology information regulation of protein monoubiquitination / Signaling by cytosolic PDGFRA and PDGFRB fusion proteins / deubiquitinase activator activity / skeletal system morphogenesis / skin development / positive regulation of double-strand break repair via homologous recombination / seminiferous tubule development / single fertilization / homeostasis of number of cells / embryonic organ development ...regulation of protein monoubiquitination / Signaling by cytosolic PDGFRA and PDGFRB fusion proteins / deubiquitinase activator activity / skeletal system morphogenesis / skin development / positive regulation of double-strand break repair via homologous recombination / seminiferous tubule development / single fertilization / homeostasis of number of cells / embryonic organ development / ubiquitin binding / positive regulation of epithelial cell proliferation / Recognition of DNA damage by PCNA-containing replication complex / Fanconi Anemia Pathway / double-strand break repair via homologous recombination / positive regulation of receptor signaling pathway via JAK-STAT / multicellular organism growth / late endosome / single-stranded DNA binding / double-stranded DNA binding / spermatogenesis / lysosome / Ub-specific processing proteases / intracellular membrane-bounded organelle / DNA damage response / DNA binding / nucleoplasm / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 3 Å | ||||||
Authors | HARRIS, S.F. / YIN, J. | ||||||
Citation | Journal: Structure / Year: 2015 Title: Structural Insights into WD-Repeat 48 Activation of Ubiquitin-Specific Protease 46. Authors: Yin, J. / Schoeffler, A.J. / Wickliffe, K. / Newton, K. / Starovasnik, M.A. / Dueber, E.C. / Harris, S.F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5cvl.cif.gz | 124 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5cvl.ent.gz | 93.3 KB | Display | PDB format |
PDBx/mmJSON format | 5cvl.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cv/5cvl ftp://data.pdbj.org/pub/pdb/validation_reports/cv/5cvl | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 67150.906 Da / Num. of mol.: 1 / Fragment: UNP residues 2-580 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: WDR48, KIAA1449, UAF1 / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: Q8TAF3 | ||||
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#2: Chemical | #3: Chemical | ChemComp-AU / #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.26 Å3/Da / Density % sol: 71.12 % / Mosaicity: 0.359 ° |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 0.72 M sodium phosphate, 0.72 M potassium phosphate, 90 mM HEPES pH 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL7-1 / Wavelength: 1.026382 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Aug 1, 2012 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Monochromator: DOUBLE CRYSTAL Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1.026382 Å / Relative weight: 1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 3→50 Å / Num. obs: 44419 / % possible obs: 100 % / Redundancy: 8.6 % / Biso Wilson estimate: 81.5 Å2 / Rmerge(I) obs: 0.123 / Χ2: 1.069 / Net I/av σ(I): 19.429 / Net I/σ(I): 7.3 / Num. measured all: 381541 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1 / Rejects: 0 / % possible all: 100
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-Phasing
Phasing | Method: SAD |
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-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 3→49.33 Å / Cor.coef. Fo:Fc: 0.9324 / Cor.coef. Fo:Fc free: 0.8988 / SU R Cruickshank DPI: 0.455 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.475 / SU Rfree Blow DPI: 0.297 / SU Rfree Cruickshank DPI: 0.297
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Displacement parameters | Biso max: 175.41 Å2 / Biso mean: 69.95 Å2 / Biso min: 16.53 Å2
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Refine analyze | Luzzati coordinate error obs: 0.378 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 3→49.33 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3→3.13 Å / Total num. of bins used: 12
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