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Yorodumi- PDB-5c7o: Structure of human testis-specific glyceraldehyde-3-phosphate deh... -
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-Basic information
Entry | Database: PDB / ID: 5c7o | ||||||
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Title | Structure of human testis-specific glyceraldehyde-3-phosphate dehydrogenase holo form with NAD+ | ||||||
Components | Glyceraldehyde-3-phosphate dehydrogenase, testis-specificGlyceraldehyde 3-phosphate dehydrogenase | ||||||
Keywords | OXIDOREDUCTASE / human sperm GAPDH metabolic enzyme sperm specific with NAD+ cofactor | ||||||
Function / homology | Function and homology information flagellated sperm motility / glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) / glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity / Gluconeogenesis / Glycolysis / Association of TriC/CCT with target proteins during biosynthesis / positive regulation of glycolytic process / glycolytic process / glucose metabolic process / NAD binding ...flagellated sperm motility / glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) / glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity / Gluconeogenesis / Glycolysis / Association of TriC/CCT with target proteins during biosynthesis / positive regulation of glycolytic process / glycolytic process / glucose metabolic process / NAD binding / NADP binding / nucleus / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.729 Å | ||||||
Authors | Betts, L. / Machius, M. / Danshina, P. / O'Brien, D. | ||||||
Citation | Journal: Mol. Hum. Reprod. / Year: 2016 Title: Structural analyses to identify selective inhibitors of glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme. Authors: Danshina, P.V. / Qu, W. / Temple, B.R. / Rojas, R.J. / Miley, M.J. / Machius, M. / Betts, L. / O'Brien, D.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5c7o.cif.gz | 270.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5c7o.ent.gz | 219.9 KB | Display | PDB format |
PDBx/mmJSON format | 5c7o.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/c7/5c7o ftp://data.pdbj.org/pub/pdb/validation_reports/c7/5c7o | HTTPS FTP |
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-Related structure data
Related structure data | 5c7iC 5c7lC 3h9eS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 36667.746 Da / Num. of mol.: 2 / Fragment: unp residues 74-407 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: GAPDHS, GAPD2, GAPDH2, GAPDS, HSD-35, HSD35 / Production host: Escherichia coli (E. coli) References: UniProt: O14556, glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 48.74 % |
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Crystal grow | Temperature: 273 K / Method: vapor diffusion Details: 1:1 mixture of protein:NAD+ complex at 10 mg/mL with 0.2 M sodium nitrate, 20% PEG3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1.0746 Å |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Apr 10, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0746 Å / Relative weight: 1 |
Reflection | Resolution: 1.73→31.71 Å / Num. obs: 68115 / % possible obs: 93.9 % / Redundancy: 4.2 % / Net I/σ(I): 19.2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3H9E Resolution: 1.729→31.706 Å / SU ML: 0.15 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 16.51 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 96.93 Å2 / Biso mean: 26.8695 Å2 / Biso min: 10.59 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.729→31.706 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 14
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