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- PDB-4zcr: Crystal structure of the C-terminal catalytic domain of Plasmodiu... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4zcr | ||||||
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Title | Crystal structure of the C-terminal catalytic domain of Plasmodium falciparum CTP:phosphocholine cytidylyltransferase in complex with phosphocholine | ||||||
![]() | Cholinephosphate cytidylyltransferase | ||||||
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Function / homology | ![]() Synthesis of PC / ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Guca, E. / Hoh, F. / Guichou, J.-F. / Cerdan, R. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural determinants of the catalytic mechanism of Plasmodium CCT, a key enzyme of malaria lipid biosynthesis. Authors: Guca, E. / Nagy, G.N. / Hajdu, F. / Marton, L. / Izrael, R. / Hoh, F. / Yang, Y. / Vial, H. / Vertessy, B.G. / Guichou, J.F. / Cerdan, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 73.5 KB | Display | ![]() |
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PDB format | ![]() | 53.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4zcpC ![]() 4zcqC ![]() 4zcsC ![]() 4zctSC C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 20810.123 Da / Num. of mol.: 1 / Fragment: UNP residues 581-775 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Strain: isolate 3D7 / Gene: ctP, MAL13P1.86 / Production host: ![]() ![]() ![]() References: UniProt: Q8IEE9, ![]() |
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#2: Chemical | ChemComp-PC / ![]() |
#3: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.49 Å3/Da / Density % sol: 50.66 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8 / Details: 20% PEG 4k |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Mar 14, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.8→46.51 Å / Num. obs: 18719 / % possible obs: 99.63 % / Redundancy: 6.4 % / Rsym value: 0.054 / Net I/σ(I): 14.3 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 4ZCT Resolution: 1.8→46.51 Å / Cor.coef. Fo:Fc: 0.968 / Cor.coef. Fo:Fc free: 0.949 / SU B: 5.966 / SU ML: 0.08 / Cross valid method: THROUGHOUT / ESU R: 0.12 / ESU R Free: 0.106 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 47.198 Å2
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Refinement step | Cycle: 1 / Resolution: 1.8→46.51 Å
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Refine LS restraints |
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