+Open data
-Basic information
Entry | Database: PDB / ID: 4wvr | ||||||
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Title | Crystal structure of Dscam1 Ig7 domain, isoform 5 | ||||||
Components | Down syndrome cell adhesion molecule, isoform AK | ||||||
Keywords | CELL ADHESION / Ig fold | ||||||
Function / homology | Function and homology information mushroom body development / detection of mechanical stimulus involved in sensory perception of touch / detection of molecule of bacterial origin / central nervous system morphogenesis / ventral cord development / dendrite self-avoidance / axon extension involved in axon guidance / axon guidance receptor activity / peripheral nervous system development / axonal fasciculation ...mushroom body development / detection of mechanical stimulus involved in sensory perception of touch / detection of molecule of bacterial origin / central nervous system morphogenesis / ventral cord development / dendrite self-avoidance / axon extension involved in axon guidance / axon guidance receptor activity / peripheral nervous system development / axonal fasciculation / regulation of dendrite morphogenesis / regulation of axonogenesis / plasma membrane => GO:0005886 / neuron development / phagocytosis / antigen binding / axon guidance / cell adhesion / neuron projection / axon / neuronal cell body / dendrite / protein homodimerization activity / identical protein binding Similarity search - Function | ||||||
Biological species | Drosophila melanogaster (fruit fly) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.948 Å | ||||||
Authors | Chen, Q. / Yu, Y. / Li, S. / Cheng, L. | ||||||
Citation | Journal: Sci Adv / Year: 2016 Title: Structural basis of Dscam1 homodimerization: Insights into context constraint for protein recognition Authors: Li, S.A. / Cheng, L. / Yu, Y. / Chen, Q. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4wvr.cif.gz | 90.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4wvr.ent.gz | 69.7 KB | Display | PDB format |
PDBx/mmJSON format | 4wvr.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wv/4wvr ftp://data.pdbj.org/pub/pdb/validation_reports/wv/4wvr | HTTPS FTP |
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-Related structure data
Related structure data | 4x5lC 4x83C 4x8xC 4x9bC 4x9fC 4x9gC 4x9hC 4x9iC 4xb7C 4xb8C 4xhqC 3dmkS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 10708.091 Da / Num. of mol.: 4 / Fragment: UNP residues 617-713 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Drosophila melanogaster (fruit fly) / Gene: Dscam1, Dscam, CG17800, Dmel_CG17800 / Production host: Escherichia coli (E. coli) / References: UniProt: Q0E9K5, UniProt: Q0E9H6*PLUS #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.48 Å3/Da / Density % sol: 50.36 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 50mM Calcium chloride dihydrate, 30%(w/v) polyethylene glycol monomethyl ether 550, 0.1M Bis-Tris PH6.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: BSRF / Beamline: 3W1A / Wavelength: 1 Å |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Jul 12, 2014 |
Radiation | Monochromator: Si(111) double-crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.948→50 Å / Num. obs: 30014 / % possible obs: 99.8 % / Redundancy: 3.8 % / Net I/σ(I): 17.7 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3DMK Resolution: 1.948→28.912 Å / SU ML: 0.21 / Cross valid method: FREE R-VALUE / σ(F): 1.98 / Phase error: 22.94 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.948→28.912 Å
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Refine LS restraints |
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LS refinement shell |
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