+Open data
-Basic information
Entry | Database: PDB / ID: 4qcj | ||||||
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Title | Crystal Structure of OdhI from Corynebacterium glutamicum | ||||||
Components | Oxoglutarate dehydrogenase inhibitor | ||||||
Keywords | PROTEIN BINDING / FHA-domain / Signal transduction / ODHA | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Corynebacterium glutamicum (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Labahn, J. / Raasch, K. / Eggeling, L. / Bocola, M. / Leitner, A. / Bott, M. | ||||||
Citation | Journal: J.Biotechnol. / Year: 2014 Title: Interaction of 2-oxoglutarate dehydrogenase OdhA with its inhibitor OdhI in Corynebacterium glutamicum: Mutants and a model. Authors: Raasch, K. / Bocola, M. / Labahn, J. / Leitner, A. / Eggeling, L. / Bott, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4qcj.cif.gz | 34.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4qcj.ent.gz | 23.1 KB | Display | PDB format |
PDBx/mmJSON format | 4qcj.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/qc/4qcj ftp://data.pdbj.org/pub/pdb/validation_reports/qc/4qcj | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 15417.016 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Corynebacterium glutamicum (bacteria) Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025 Gene: cg1630, Cgl1441, odhI / Production host: Escherichia coli (E. coli) / References: UniProt: Q8NQJ3 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.51 Å3/Da / Density % sol: 18.31 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion / pH: 7.5 Details: 17% PEG 3350, 0.2 M Sodium malonate, 0.1 M Hepes, pH 7.5, VAPOR DIFFUSION, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-1 / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Sep 13, 2009 |
Radiation | Monochromator: diamond / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2→51 Å / Num. all: 6688 / Num. obs: 6634 / % possible obs: 99.2 % / Observed criterion σ(F): 20894 / Observed criterion σ(I): 2 |
Reflection shell | Resolution: 2→2.11 Å / % possible all: 99.7 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2→29.19 Å / SU ML: 0.17 / σ(F): 1.38 / Phase error: 21.24 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→29.19 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 35.0905 Å / Origin y: 11.7625 Å / Origin z: 17.6888 Å
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Refinement TLS group | Selection details: all |