+Open data
-Basic information
Entry | Database: PDB / ID: 2cbp | |||||||||
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Title | CUCUMBER BASIC PROTEIN, A BLUE COPPER PROTEIN | |||||||||
Components | CUCUMBER BASIC PROTEIN | |||||||||
Keywords | ELECTRON TRANSPORT / PHYTOCYANIN / TYPE 1 COPPER PROTEIN | |||||||||
Function / homology | Function and homology information pollination / anther development / apoplast / extracellular matrix / electron transfer activity / metal ion binding Similarity search - Function | |||||||||
Biological species | Cucumis sativus (cucumber) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 1.8 Å | |||||||||
Authors | Guss, J.M. / Freeman, H.C. | |||||||||
Citation | Journal: J.Mol.Biol. / Year: 1996 Title: The structure of a phytocyanin, the basic blue protein from cucumber, refined at 1.8 A resolution. Authors: Guss, J.M. / Merritt, E.A. / Phizackerley, R.P. / Freeman, H.C. #1: Journal: Science / Year: 1988 Title: Phase Determination by Multiple-Wavelength X-Ray Diffraction. Crystal Structure of a Basic 'Blue' Copper Protein from Cucumbers Authors: Guss, J.M. / Merritt, E.A. / Phizackerley, R.P. / Hedman, B. / Murata, M. / Hodgson, K.O. / Freeman, H.C. #2: Journal: J.Mol.Biol. / Year: 1977 Title: Preliminary Crystallographic Data for a Basic Copper-Containing Protein from Cucumber Seedlings Authors: Colman, P.M. / Freeman, H.C. / Guss, J.M. / Murata, M. / Norris, V.A. / Ramshaw, J.A. / Venkatappa, M.P. / Vickery, L.E. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2cbp.cif.gz | 51.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2cbp.ent.gz | 37.7 KB | Display | PDB format |
PDBx/mmJSON format | 2cbp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cb/2cbp ftp://data.pdbj.org/pub/pdb/validation_reports/cb/2cbp | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 10371.791 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: FROM SEEDLINGS / Source: (natural) Cucumis sativus (cucumber) / References: UniProt: P00303 |
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#2: Chemical | ChemComp-CU / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.26 Å3/Da / Density % sol: 47 % | |||||||||||||||
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Crystal grow | *PLUS pH: 6 / Method: vapor diffusion, hanging drop | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL1-5 / Wavelength: 1.2359, 1.3771, 1.3790, 1.5416 | |||||||||||||||
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Detector | Type: XUONG-HAMLIN MULTIWIRE / Detector: AREA DETECTOR / Date: 1986 | |||||||||||||||
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength |
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Reflection | Num. obs: 43466 / % possible obs: 89 % / Observed criterion σ(I): 1 / Rmerge(I) obs: 0.043 |
-Processing
Software | Name: PROLSQ / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Resolution: 1.8→7 Å / σ(F): 0.5 Details: 1988 ESTIMATED COORD. ERROR 0.12 ANGSTROMS (BACKBONE) ESTIMATED COORD. ERROR 0.17 ANGSTROMS (SIDE CHAIN)
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Displacement parameters | Biso mean: 11.9 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.15 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→7 Å
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Refine LS restraints |
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Refinement | *PLUS Rfactor obs: 0.141 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |