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- PDB-4moi: Pyranose 2-oxidase H450G/V546C double mutant with 3-fluorinated g... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4moi | ||||||
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Title | Pyranose 2-oxidase H450G/V546C double mutant with 3-fluorinated glucose | ||||||
![]() | Pyranose 2-oxidase | ||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Tan, T.C. / Spadiut, O. / Gandini, R. / Haltrich, D. / Divne, C. | ||||||
![]() | ![]() Title: Structural Basis for Binding of Fluorinated Glucose and Galactose to Trametes multicolor Pyranose 2-Oxidase Variants with Improved Galactose Conversion. Authors: Tan, T.C. / Spadiut, O. / Gandini, R. / Haltrich, D. / Divne, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 456.4 KB | Display | ![]() |
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PDB format | ![]() | 380.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4moeC ![]() 4mofC ![]() 4mogC ![]() 4mohC ![]() 4mojC ![]() 4mokC ![]() 4molC ![]() 4momC ![]() 4mooC ![]() 4mopC ![]() 4moqC ![]() 4morC ![]() 4mosC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 70476.969 Da / Num. of mol.: 2 / Mutation: H450G, V546C Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() #2: Chemical | #3: Sugar | #4: Water | ChemComp-HOH / | ![]() Sequence details | THIS IS A CLONING ARTIFACT | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.18 Å3/Da / Density % sol: 43.6 % |
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Crystal grow![]() | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 5.2 Details: 0.1 M MES, 50 mM MgCl2, 10% (w/v) monomethylether PEG 2000, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Feb 12, 2013 / Details: unfocused |
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.9→47.73 Å / Num. all: 98867 / Num. obs: 98867 / % possible obs: 100 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 10.9 % / Net I/σ(I): 10.8 |
Reflection shell | Resolution: 1.9→2 Å / Redundancy: 10.3 % / Mean I/σ(I) obs: 2 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 26.8 Å2
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Refinement step | Cycle: LAST / Resolution: 1.9→45.88 Å
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Refine LS restraints |
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