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Yorodumi- PDB-4l2b: X-ray structure of the C57S mutant of the iron superoxide dismuta... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4l2b | |||||||||
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Title | X-ray structure of the C57S mutant of the iron superoxide dismutase from Pseudoalteromonas haloplanktis | |||||||||
Components | Superoxide dismutase [Fe] | |||||||||
Keywords | OXIDOREDUCTASE / Superoxide dismutase / C57S mutant | |||||||||
Function / homology | Function and homology information superoxide dismutase / superoxide dismutase activity / metal ion binding Similarity search - Function | |||||||||
Biological species | Pseudoalteromonas haloplanktis (bacteria) | |||||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.97 Å | |||||||||
Authors | Merlino, A. / Russo Krauss, I. / Sica, F. | |||||||||
Citation | Journal: Biochim.Biophys.Acta / Year: 2014 Title: Structural and denaturation studies of two mutants of a cold adapted superoxide dismutase point to the importance of electrostatic interactions in protein stability. Authors: Merlino, A. / Russo Krauss, I. / Castellano, I. / Ruocco, M.R. / Capasso, A. / De Vendittis, E. / Rossi, B. / Sica, F. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4l2b.cif.gz | 97 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4l2b.ent.gz | 72.9 KB | Display | PDB format |
PDBx/mmJSON format | 4l2b.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/l2/4l2b ftp://data.pdbj.org/pub/pdb/validation_reports/l2/4l2b | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 21253.443 Da / Num. of mol.: 2 / Mutation: C57S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudoalteromonas haloplanktis (bacteria) Gene: sodB, PSHAa1215 / Production host: Escherichia coli (E. coli) / References: UniProt: P84612, superoxide dismutase #2: Polysaccharide | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.73 Å3/Da / Density % sol: 54.98 % |
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Crystal grow | Temperature: 293 K / pH: 7.2 Details: Ammonium sulphate 2.0 M, NaCl 0.1 M, Hepes 0.1 M, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU MICROMAX-007 HF / Wavelength: 1.54 |
Detector | Type: RIGAKU SATURN 944 / Detector: CCD / Date: May 11, 2007 / Details: MIRRORS |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 1.97→27.5 Å / Num. obs: 30651 / % possible obs: 95 % / Observed criterion σ(I): 0 / Redundancy: 3.5 % / Rmerge(I) obs: 0.099 / Net I/σ(I): 9 |
Reflection shell | Resolution: 1.97→2.04 Å / Redundancy: 2.8 % / Rmerge(I) obs: 0.269 / Mean I/σ(I) obs: 3 / % possible all: 90 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.97→27.5 Å / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 1.97→27.5 Å
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