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Yorodumi- PDB-4l2a: X-ray structure of the C57R mutant of the iron superoxide dismuta... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4l2a | ||||||
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Title | X-ray structure of the C57R mutant of the iron superoxide dismutase from Pseudoalteromonas haloplanktis (crystal form II) | ||||||
Components | Superoxide dismutase [Fe] | ||||||
Keywords | OXIDOREDUCTASE / Superoxide dismutase / C57R mutant | ||||||
Function / homology | Function and homology information superoxide dismutase / superoxide dismutase activity / metal ion binding Similarity search - Function | ||||||
Biological species | Pseudoalteromonas haloplanktis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.06 Å | ||||||
Authors | Merlino, A. / Russo Krauss, I. / Sica, F. | ||||||
Citation | Journal: Biochim.Biophys.Acta / Year: 2014 Title: Structural and denaturation studies of two mutants of a cold adapted superoxide dismutase point to the importance of electrostatic interactions in protein stability. Authors: Merlino, A. / Russo Krauss, I. / Castellano, I. / Ruocco, M.R. / Capasso, A. / De Vendittis, E. / Rossi, B. / Sica, F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4l2a.cif.gz | 93.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4l2a.ent.gz | 70.9 KB | Display | PDB format |
PDBx/mmJSON format | 4l2a.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/l2/4l2a ftp://data.pdbj.org/pub/pdb/validation_reports/l2/4l2a | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 21323.561 Da / Num. of mol.: 2 / Mutation: C57R Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudoalteromonas haloplanktis (bacteria) Gene: sodB, PSHAa1215 / Production host: Escherichia coli (E. coli) / References: UniProt: P84612, superoxide dismutase #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.65 Å3/Da / Density % sol: 53.53 % |
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Crystal grow | Temperature: 293 K / pH: 7 Details: PEG 8K 23%, ammonium sulphate 0.2 M, sodium cacodilate 0.1 M, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ELETTRA / Beamline: 5.2R / Wavelength: 1 |
Detector | Date: Oct 10, 2007 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.06→28 Å / Num. obs: 27167 / % possible obs: 98.8 % / Observed criterion σ(I): 0 / Redundancy: 4 % / Rmerge(I) obs: 0.074 / Net I/σ(I): 13 |
Reflection shell | Resolution: 2.06→2.13 Å / Redundancy: 3.2 % / Rmerge(I) obs: 0.235 / Mean I/σ(I) obs: 4 / % possible all: 90.4 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.06→28 Å / σ(F): 0 / Stereochemistry target values: ENGH & HUBER
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Refinement step | Cycle: LAST / Resolution: 2.06→28 Å
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