+Open data
-Basic information
Entry | Database: PDB / ID: 4gr6 | ||||||
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Title | Crystal structure of AtRbcX2 from Arabidopsis thaliana | ||||||
Components | AtRbcX2 | ||||||
Keywords | CHAPERONE / Rubisco / RbcX | ||||||
Function / homology | Function and homology information chloroplast stroma / chaperone-mediated protein folding / protein folding chaperone Similarity search - Function | ||||||
Biological species | Arabidopsis thaliana (thale cress) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Grudnik, P. / Golik, P. / Kolesinski, P. / Dubin, G. / Szczepaniak, A. | ||||||
Citation | Journal: Biochim.Biophys.Acta / Year: 2013 Title: Insights into eukaryotic Rubisco assembly - Crystal structures of RbcX chaperones from Arabidopsis thaliana. Authors: Kolesinski, P. / Golik, P. / Grudnik, P. / Piechota, J. / Markiewicz, M. / Tarnawski, M. / Dubin, G. / Szczepaniak, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4gr6.cif.gz | 105 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4gr6.ent.gz | 81.2 KB | Display | PDB format |
PDBx/mmJSON format | 4gr6.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gr/4gr6 ftp://data.pdbj.org/pub/pdb/validation_reports/gr/4gr6 | HTTPS FTP |
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-Related structure data
Related structure data | 4gr2C 2z44S C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 15039.025 Da / Num. of mol.: 2 / Fragment: UNP residues 79-203 / Mutation: C91A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Gene: At5g19855 / Production host: Escherichia coli (E. coli) / References: UniProt: Q8L9X2 #2: Chemical | ChemComp-EDO / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.75 Å3/Da / Density % sol: 55.26 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, sitting drop / pH: 5.6 Details: 15% PEG 400, 100 mM sodium citrate , pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU MICROMAX-007 HF / Wavelength: 1.54 Å |
Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: May 21, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 2→33.75 Å / Num. all: 21808 / Num. obs: 21808 / % possible obs: 100 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2Z44 Resolution: 2→21.167 Å / SU ML: 0.16 / σ(F): 1.35 / Phase error: 21.03 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→21.167 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: -12.1395 Å / Origin y: 19.2645 Å / Origin z: -14.6886 Å
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Refinement TLS group | Selection details: all |