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Yorodumi- PDB-2mkh: Solution structure of tandem RRM domains of cytoplasmic polyadeny... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2mkh | ||||||
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Title | Solution structure of tandem RRM domains of cytoplasmic polyadenylation element binding protein 1 (CPEB1) in free state | ||||||
Components | Cytoplasmic polyadenylation element-binding protein 1 | ||||||
Keywords | TRANSLATION REGULATOR / CPEB1 / RNA recognition motif (RRM) / Cytoplasmic polyadenylation / Translational regulation | ||||||
Function / homology | Function and homology information messenger ribonucleoprotein complex / regulation of mRNA 3'-end processing / translation factor activity, RNA binding / mRNA 3'-UTR AU-rich region binding / negative regulation of cytoplasmic translation / mRNA regulatory element binding translation repressor activity / mRNA 3'-UTR binding / cellular response to amino acid stimulus / P-body / mRNA processing ...messenger ribonucleoprotein complex / regulation of mRNA 3'-end processing / translation factor activity, RNA binding / mRNA 3'-UTR AU-rich region binding / negative regulation of cytoplasmic translation / mRNA regulatory element binding translation repressor activity / mRNA 3'-UTR binding / cellular response to amino acid stimulus / P-body / mRNA processing / cellular response to insulin stimulus / ribosome binding / cellular response to hypoxia / postsynaptic density / neuron projection / synapse / dendrite / nucleoplasm / membrane / metal ion binding / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | closest to the average, model2 | ||||||
Authors | Afroz, T. / Skrisovska, L. / Belloc, E. / Boixet, J.G. / Mendez, R. / Allain, F.H.-T. | ||||||
Citation | Journal: Genes Dev. / Year: 2014 Title: A fly trap mechanism provides sequence-specific RNA recognition by CPEB proteins Authors: Afroz, T. / Skrisovska, L. / Belloc, E. / Guillen-Boixet, J. / Mendez, R. / Allain, F.H.-T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2mkh.cif.gz | 1.3 MB | Display | PDBx/mmCIF format |
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PDB format | pdb2mkh.ent.gz | 1.1 MB | Display | PDB format |
PDBx/mmJSON format | 2mkh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mk/2mkh ftp://data.pdbj.org/pub/pdb/validation_reports/mk/2mkh | HTTPS FTP |
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-Related structure data
Related structure data | 2mkeC 2mkiC 2mkjC 2mkkC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 24375.020 Da / Num. of mol.: 1 / Fragment: UNP residues 219-434 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CPEB1, CPEB / Production host: Escherichia coli (E. coli) / References: UniProt: Q9BZB8 |
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Sequence details | THIS SEQUENCE IS ISOFORM 4 OF CPEB1_HUMAN. |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 100 mM sodium chloride-1, 1 mM DTT-2, 1 mM magnesium sulphate-3, 50 mM sodium phosphate-4, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | |||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 0.15 / pH: 6.5 / Pressure: ambient / Temperature: 303 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software | Name: Amber Developer: Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman Classification: refinement |
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Refinement | Method: simulated annealing / Software ordinal: 1 |
NMR representative | Selection criteria: closest to the average |
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 30 / Conformers submitted total number: 20 / Representative conformer: 2 |