+Open data
-Basic information
Entry | Database: PDB / ID: 3wcy | ||||||
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Title | Murine Ifnar1 in complex with interferon-beta | ||||||
Components |
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Keywords | CYTOKINE RECEPTOR/CYTOKINE / Fibronectin Type III / Helical cytokine / Cytokine receptor / Interferon / CYTOKINE RECEPTOR-CYTOKINE complex | ||||||
Function / homology | Function and homology information negative regulation of Lewy body formation / interferon receptor activity / type I interferon production / negative regulation of matrix metallopeptidase secretion / negative regulation of mononuclear cell migration / type I interferon receptor activity / type I interferon binding / Regulation of IFNA/IFNB signaling / type I interferon receptor binding / negative regulation of immunoglobulin production ...negative regulation of Lewy body formation / interferon receptor activity / type I interferon production / negative regulation of matrix metallopeptidase secretion / negative regulation of mononuclear cell migration / type I interferon receptor activity / type I interferon binding / Regulation of IFNA/IFNB signaling / type I interferon receptor binding / negative regulation of immunoglobulin production / Interferon alpha/beta signaling / cellular response to interferon-alpha / natural killer cell activation involved in immune response / negative regulation of blood-brain barrier permeability / positive regulation of peptidyl-serine phosphorylation of STAT protein / negative regulation of neuroinflammatory response / negative regulation of cell adhesion molecule production / positive regulation of transforming growth factor beta production / T cell activation involved in immune response / macrophage activation involved in immune response / cellular response to dsRNA / cytokine receptor activity / type I interferon-mediated signaling pathway / response to exogenous dsRNA / B cell proliferation / negative regulation of osteoclast differentiation / plasma membrane => GO:0005886 / humoral immune response / negative regulation of type II interferon production / positive regulation of autophagy / regulation of peptidyl-tyrosine phosphorylation / T cell activation / positive regulation of interferon-beta production / B cell differentiation / cellular response to dexamethasone stimulus / positive regulation of interleukin-1 beta production / cytokine activity / cellular response to virus / cytokine-mediated signaling pathway / neuron cellular homeostasis / positive regulation of type II interferon production / late endosome / defense response to virus / adaptive immune response / response to lipopolysaccharide / membrane => GO:0016020 / lysosome / defense response to bacterium / negative regulation of cell population proliferation / positive regulation of DNA-templated transcription / extracellular space / extracellular region / plasma membrane Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.9 Å | ||||||
Authors | Vivian, J.P. / de Weerd, N.A. / Hertzog, P.J. / Rossjohn, J. | ||||||
Citation | Journal: To be Published Title: Structural basis of a unique interferon beta signaling axis mediated via the IFNAR1 receptor Authors: de Weerd, N.A. / Vivian, J.P. / Nguyen, T.K. / Mangan, N.E. / Gould, J.A. / Braniff, S.J. / Zaker-Tabrizi, L. / Fung, K.Y. / Forster, S.C. / Beddoe, T. / Reid, H.H. / Rossjohn, J. / Hertzog, P.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3wcy.cif.gz | 211.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3wcy.ent.gz | 171.6 KB | Display | PDB format |
PDBx/mmJSON format | 3wcy.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wc/3wcy ftp://data.pdbj.org/pub/pdb/validation_reports/wc/3wcy | HTTPS FTP |
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-Related structure data
Related structure data | 3se4S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 45805.461 Da / Num. of mol.: 1 / Fragment: extracellular domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ifar, Ifnar, Ifnar1 / Production host: Spodoptera frugiperda (fall armyworm) / Strain (production host): SF9 / References: UniProt: P33896 |
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#2: Protein | Mass: 19760.926 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Ifb, Ifnb, Ifnb1 / Production host: Spodoptera frugiperda (fall armyworm) / Strain (production host): SF9 / References: UniProt: P01575 |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.72 Å3/Da / Density % sol: 54.86 % |
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Crystal grow | Temperature: 297 K / Method: vapor diffusion, hanging drop / pH: 6 Details: 12% PEG 3350, 8% tacsimate pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Australian Synchrotron / Beamline: MX2 / Wavelength: 0.9436 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Oct 5, 2012 |
Radiation | Monochromator: synchrotron / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9436 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→50 Å / Num. all: 15228 / Num. obs: 15228 / % possible obs: 98.7 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 4 % / Biso Wilson estimate: 103.66 Å2 / Rmerge(I) obs: 0.064 / Net I/σ(I): 11.7 |
Reflection shell | Resolution: 2.9→3.06 Å / Redundancy: 4.1 % / Rmerge(I) obs: 0.55 / Mean I/σ(I) obs: 2.4 / Num. unique all: 2254 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3SE4 Resolution: 2.9→17.99 Å / Cor.coef. Fo:Fc: 0.9017 / Cor.coef. Fo:Fc free: 0.8746 / Cross valid method: THROUGHOUT / σ(F): 0
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Displacement parameters | Biso mean: 113 Å2
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Refine analyze | Luzzati coordinate error obs: 0.608 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.9→17.99 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.9→3.1 Å / Total num. of bins used: 8
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Refinement TLS params. | T13: -0.152 Å2 / Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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