+Open data
-Basic information
Entry | Database: PDB / ID: 3omv | |||||||||
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Title | Crystal structure of c-raf (raf-1) | |||||||||
Components | RAF proto-oncogene serine/threonine-protein kinase | |||||||||
Keywords | TRANSFERASE / SERINE/THREONINE-PROTEIN KINASE | |||||||||
Function / homology | Function and homology information death-inducing signaling complex assembly / intermediate filament cytoskeleton organization / type B pancreatic cell proliferation / regulation of Rho protein signal transduction / SHOC2 M1731 mutant abolishes MRAS complex function / Gain-of-function MRAS complexes activate RAF signaling / Rap1 signalling / regulation of cell motility / insulin secretion involved in cellular response to glucose stimulus / Negative feedback regulation of MAPK pathway ...death-inducing signaling complex assembly / intermediate filament cytoskeleton organization / type B pancreatic cell proliferation / regulation of Rho protein signal transduction / SHOC2 M1731 mutant abolishes MRAS complex function / Gain-of-function MRAS complexes activate RAF signaling / Rap1 signalling / regulation of cell motility / insulin secretion involved in cellular response to glucose stimulus / Negative feedback regulation of MAPK pathway / IFNG signaling activates MAPKs / GP1b-IX-V activation signalling / ERBB2-ERBB3 signaling pathway / regulation of cell differentiation / face development / pseudopodium / somatic stem cell population maintenance / neurotrophin TRK receptor signaling pathway / thyroid gland development / extrinsic apoptotic signaling pathway via death domain receptors / MAP kinase kinase kinase activity / negative regulation of protein-containing complex assembly / Schwann cell development / type II interferon-mediated signaling pathway / negative regulation of extrinsic apoptotic signaling pathway via death domain receptors / activation of adenylate cyclase activity / response to muscle stretch / myelination / CD209 (DC-SIGN) signaling / insulin-like growth factor receptor signaling pathway / thymus development / RAF activation / Signaling by high-kinase activity BRAF mutants / MAP2K and MAPK activation / wound healing / negative regulation of cysteine-type endopeptidase activity involved in apoptotic process / Stimuli-sensing channels / Negative regulation of MAPK pathway / Signaling by RAF1 mutants / Signaling by moderate kinase activity BRAF mutants / Paradoxical activation of RAF signaling by kinase inactive BRAF / Signaling downstream of RAS mutants / MAPK cascade / Signaling by BRAF and RAF1 fusions / insulin receptor signaling pathway / positive regulation of peptidyl-serine phosphorylation / regulation of apoptotic process / mitochondrial outer membrane / positive regulation of MAPK cascade / non-specific serine/threonine protein kinase / protein kinase activity / negative regulation of cell population proliferation / protein phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / apoptotic process / negative regulation of apoptotic process / Golgi apparatus / enzyme binding / signal transduction / positive regulation of transcription by RNA polymerase II / mitochondrion / ATP binding / identical protein binding / metal ion binding / nucleus / plasma membrane / cytosol / cytoplasm Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 4 Å | |||||||||
Authors | Hatzivassiliou, G. / Song, K. / Yen, I. / Brandhuber, B.J. / Anderson, D.J. / Alvarado, R. / Ludlam, M.J. / Stokoe, D. / Gloor, S.L. / Vigers, G.P.A. ...Hatzivassiliou, G. / Song, K. / Yen, I. / Brandhuber, B.J. / Anderson, D.J. / Alvarado, R. / Ludlam, M.J. / Stokoe, D. / Gloor, S.L. / Vigers, G.P.A. / Morales, T. / Aliagas, I. / Liu, B. / Sideris, S. / Hoeflich, K.P. / Jaiswal, B.S. / Seshagiri, S. / Koeppen, H. / Belvin, M. / Friedman, L.S. / Malek, S. | |||||||||
Citation | Journal: Nature / Year: 2010 Title: RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. Authors: Hatzivassiliou, G. / Song, K. / Yen, I. / Brandhuber, B.J. / Anderson, D.J. / Alvarado, R. / Ludlam, M.J. / Stokoe, D. / Gloor, S.L. / Vigers, G. / Morales, T. / Aliagas, I. / Liu, B. / ...Authors: Hatzivassiliou, G. / Song, K. / Yen, I. / Brandhuber, B.J. / Anderson, D.J. / Alvarado, R. / Ludlam, M.J. / Stokoe, D. / Gloor, S.L. / Vigers, G. / Morales, T. / Aliagas, I. / Liu, B. / Sideris, S. / Hoeflich, K.P. / Jaiswal, B.S. / Seshagiri, S. / Koeppen, H. / Belvin, M. / Friedman, L.S. / Malek, S. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3omv.cif.gz | 106.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3omv.ent.gz | 79.3 KB | Display | PDB format |
PDBx/mmJSON format | 3omv.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/om/3omv ftp://data.pdbj.org/pub/pdb/validation_reports/om/3omv | HTTPS FTP |
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-Related structure data
Related structure data | 3d4qS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
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-Components
#1: Protein | Mass: 35430.727 Da / Num. of mol.: 2 / Fragment: C-RAF kinase domain, UNP residues 323-618 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: RAF1, RAF / Production host: Spodoptera frugiperda (fall armyworm) References: UniProt: P04049, non-specific serine/threonine protein kinase #2: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.82 Å3/Da / Density % sol: 56.43 % |
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Crystal grow | Temperature: 285 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 12% PEG 8K, 100 MM TRIS PH 8.0, 10% TACSIMATE, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU FR-E SUPERBRIGHT / Wavelength: 1.5418 Å |
Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Jan 18, 2006 |
Radiation | Monochromator: Osmic mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 4→30 Å / Num. all: 7361 / Num. obs: 7341 / % possible obs: 99.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 13.8 % / Biso Wilson estimate: 68 Å2 / Rmerge(I) obs: 0.167 / Rsym value: 0.167 / Net I/σ(I): 4.3 |
Reflection shell | Resolution: 4→4.22 Å / Redundancy: 14.2 % / Rmerge(I) obs: 0.311 / Mean I/σ(I) obs: 2.5 / Num. unique all: 1048 / Rsym value: 0.311 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3D4Q Resolution: 4→29.9 Å / Cor.coef. Fo:Fc: 0.849 / Cor.coef. Fo:Fc free: 0.682 / SU B: 69.399 / SU ML: 0.942 / Isotropic thermal model: Uniform B=68.0 / Cross valid method: THROUGHOUT / ESU R Free: 1.166 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 68 Å2
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Refinement step | Cycle: LAST / Resolution: 4→29.9 Å
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Refine LS restraints |
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Refine LS restraints NCS | Number: 2130 / Type: tight positional / Rms dev position: 0.45 Å / Weight position: 0.5 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 4→4.102 Å / Total num. of bins used: 20
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