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Open data
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Basic information
Entry | Database: PDB / ID: 3jss | ||||||
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Title | Crystal structure of a mutant RelB dimerization domain | ||||||
![]() | Transcription factor RelB | ||||||
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Function / homology | ![]() T-helper 1 cell differentiation / Dectin-1 mediated noncanonical NF-kB signaling / NIK-->noncanonical NF-kB signaling / CD209 (DC-SIGN) signaling / myeloid dendritic cell differentiation / negative regulation of interferon-beta production / cellular response to osmotic stress / T-helper 1 type immune response / non-canonical NF-kappaB signal transduction / antigen processing and presentation ...T-helper 1 cell differentiation / Dectin-1 mediated noncanonical NF-kB signaling / NIK-->noncanonical NF-kB signaling / CD209 (DC-SIGN) signaling / myeloid dendritic cell differentiation / negative regulation of interferon-beta production / cellular response to osmotic stress / T-helper 1 type immune response / non-canonical NF-kappaB signal transduction / antigen processing and presentation / canonical NF-kappaB signal transduction / transcription repressor complex / response to cytokine / circadian regulation of gene expression / DNA-binding transcription factor activity, RNA polymerase II-specific / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Vu, D. / Huang, D.B. / Ghosh, G. | ||||||
![]() | ![]() Title: A structural basis for selective dimerization by NF-kappa B RelB. Authors: Vu, D. / Huang, D.B. / Vemu, A. / Ghosh, G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 31.3 KB | Display | ![]() |
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PDB format | ![]() | 20.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 11460.842 Da / Num. of mol.: 1 / Fragment: dimerization domain (UNP residues 278-378) / Mutation: V314R, A324G, F358Q, L362K Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.94 Å3/Da / Density % sol: 68.82 % |
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Crystal grow![]() | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 20% PEG800, 0.1M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 105 K |
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Diffraction source | Source: ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Jan 1, 2006 / Details: mirros |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.6→30 Å / Num. all: 5318 / Num. obs: 4987 / % possible obs: 85 % / Observed criterion σ(I): 1 / Redundancy: 12 % / Rmerge(I) obs: 0.073 / Net I/σ(I): 14.7 |
Reflection shell | Resolution: 2.6→2.69 Å / Redundancy: 10 % / Rmerge(I) obs: 0.29 / Mean I/σ(I) obs: 2.8 / Num. unique all: 288 / % possible all: 50 |
-Phasing
Phasing![]() | Method: ![]() |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 27.682 Å2 / ksol: 0.358 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 114.1 Å2 / Biso mean: 39.386 Å2 / Biso min: 7.71 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.6→23.48 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.6→2.76 Å / Rfactor Rfree error: 0.051 / Total num. of bins used: 6
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Xplor file | Serial no: 1 / Param file: protein_rep.param / Topol file: protein.top |