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Yorodumi- PDB-2vns: Crystal Structure of the Membrane Proximal Oxidoreductase Domain ... -
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-Basic information
Entry | Database: PDB / ID: 2vns | ||||||
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Title | Crystal Structure of the Membrane Proximal Oxidoreductase Domain of Human Steap3, the Dominant Ferric Reductase of the Erythroid Transferrin Cycle | ||||||
Components | METALLOREDUCTASE STEAP3 | ||||||
Keywords | OXIDOREDUCTASE / METAL-BINDING / TRANSMEMBRANE / ROSSMANN FOLD / TRANSPORT / CELL CYCLE / TRANSFERRIN / FLAVOPROTEIN / ALTERNATIVE SPLICING / TRANSFERRIN RECEPTOR / FERRIREDUCTASE / FERRIC-REDUCTASE / IRON TRANSPORT / PHOSPHOPROTEIN / STEAP3 / COPPER / MEMBRANE / ENDOSOME / APOPTOSIS / TF / NAD / TFR / FAD / FNO / NADP / TFR1 / IRON / STEAP / POLYMORPHISM / GLYCOPROTEIN / ION TRANSPORT / DINUCLEOTIDE-BINDING DOMAIN | ||||||
Function / homology | Function and homology information Oxidoreductases; Oxidizing metal ions; With NAD+ or NADP+ as acceptor / oxidoreductase activity, acting on metal ions, NAD or NADP as acceptor / ferric-chelate reductase (NADPH) activity / cupric reductase activity / transferrin transport / copper ion import / Transferrin endocytosis and recycling / RHOF GTPase cycle / RHOD GTPase cycle / RHOJ GTPase cycle ...Oxidoreductases; Oxidizing metal ions; With NAD+ or NADP+ as acceptor / oxidoreductase activity, acting on metal ions, NAD or NADP as acceptor / ferric-chelate reductase (NADPH) activity / cupric reductase activity / transferrin transport / copper ion import / Transferrin endocytosis and recycling / RHOF GTPase cycle / RHOD GTPase cycle / RHOJ GTPase cycle / RHOQ GTPase cycle / TP53 Regulates Transcription of Genes Involved in Cytochrome C Release / CDC42 GTPase cycle / protein secretion / FAD binding / multivesicular body / endosome membrane / endosome / cell cycle / apoptotic process / heme binding / identical protein binding / metal ion binding / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | HOMO SAPIENS (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MIRAS / Resolution: 2 Å | ||||||
Authors | Sendamarai, A.K. / Ohgami, R.S. / Fleming, M.D. / Lawrence, C.M. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2008 Title: Structure of the Membrane Proximal Oxidoreductase Domain of Human Steap3, the Dominant Ferrireductase of the Erythroid Transferrin Cycle Authors: Sendamarai, A.K. / Ohgami, R.S. / Fleming, M.D. / Lawrence, C.M. #1: Journal: Nat.Genet. / Year: 2005 Title: Identification of a Ferrireductase Required for Efficient Transferrin-Dependent Iron Uptake in Erythroid Cells Authors: Ohgami, R.S. / Campagna, D.R. / Greer, E.L. / Antiochos, B. / Mcdonald, A. / Chen, J. / Sharp, J.J. / Fujiwara, Y. / Barker, J.E. / Fleming, M.D. #2: Journal: Blood / Year: 2005 Title: Nm1054, a Spontaneous, Recessive, Hypochromic, Microcytic Anemia Mutation in the Mouse Authors: Ohgami, R.S. / Campagna, D.R. / Antiochos, B. / Wood, E.B. / Sharp, J.J. / Barker, J.E. / Fleming, M.D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2vns.cif.gz | 150.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2vns.ent.gz | 122.2 KB | Display | PDB format |
PDBx/mmJSON format | 2vns.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/vn/2vns ftp://data.pdbj.org/pub/pdb/validation_reports/vn/2vns | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
NCS oper: (Code: given Matrix: (-1, 0.002, 0.013), Vector: |
-Components
#1: Protein | Mass: 23315.676 Da / Num. of mol.: 2 Fragment: NADPH/FLAVIN DEPENDENT OXIDOREDUCTASE, RESIDUES 1-215 Source method: isolated from a genetically manipulated source Details: RESIDUES 1-215 CLONED / Source: (gene. exp.) HOMO SAPIENS (human) / Tissue: KIDNEY, RENAL CELL ADENOCARCINOMA / Plasmid: PDEST14 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): RIL / References: UniProt: Q658P3, EC: 1.16.1.2 #2: Chemical | ChemComp-CIT / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 3 |
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-Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 47.01 % Description: MIRAS TECHNIQUE INCLUDED MAD FROM INCORPORATED PT AND MIR USING PT AND HG DERIVATIVES |
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Crystal grow | pH: 5.6 Details: 0.01M FECL3, 40-100MM NA3.CITRATE AND 4% V/V JEFFAMINE M600 PH5.6 |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL9-2 / Wavelength: 1.07149, 0.82654, 1.07199, 1.00545, 1.1271 | ||||||||||||||||||
Detector | Type: MARRESEARCH / Detector: CCD / Date: Mar 11, 2007 Details: FLAT COLLIMATING MIRROR, DOUBLE CRYSTAL MONOCHROMATOR, TOROID FOCUSING MIRROR | ||||||||||||||||||
Radiation | Monochromator: DOUBLE CRYSTAL MONOCHROMATOR / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||
Radiation wavelength |
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Reflection | Resolution: 1.98→50 Å / Num. obs: 25300 / % possible obs: 96.8 % / Observed criterion σ(I): 5 / Redundancy: 3.8 % / Rmerge(I) obs: 0.05 / Net I/σ(I): 16.1 | ||||||||||||||||||
Reflection shell | Resolution: 1.98→2.05 Å / Redundancy: 3.7 % / Rmerge(I) obs: 0.24 / Mean I/σ(I) obs: 3.3 / % possible all: 98.3 |
-Processing
Software |
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Refinement | Method to determine structure: MIRAS Starting model: NONE Resolution: 2→50 Å / Cor.coef. Fo:Fc: 0.949 / Cor.coef. Fo:Fc free: 0.929 / SU B: 8.467 / SU ML: 0.122 / Cross valid method: THROUGHOUT / ESU R Free: 0.177 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. DISORDERED REGION BETWEEN RESIDUES 159 TO 164 HAS POOR DENSITY FOR SIDE CHAINS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 19.24 Å2
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Refinement step | Cycle: LAST / Resolution: 2→50 Å
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Refine LS restraints |
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