+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 2ms7 | ||||||
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タイトル | High-resolution solid-state NMR structure of the helical signal transduction filament MAVS CARD | ||||||
要素 | Mitochondrial antiviral-signaling protein | ||||||
キーワード | PROTEIN BINDING (タンパク質) / MAVS CARD filament | ||||||
機能・相同性 | 機能・相同性情報 positive regulation of IP-10 production / regulation of peroxisome organization / RIG-I binding / positive regulation of chemokine (C-C motif) ligand 5 production / positive regulation of myeloid dendritic cell cytokine production / CARD domain binding / NF-kB activation through FADD/RIP-1 pathway mediated by caspase-8 and -10 / protein localization to mitochondrion / positive regulation of response to cytokine stimulus / positive regulation of type I interferon-mediated signaling pathway ...positive regulation of IP-10 production / regulation of peroxisome organization / RIG-I binding / positive regulation of chemokine (C-C motif) ligand 5 production / positive regulation of myeloid dendritic cell cytokine production / CARD domain binding / NF-kB activation through FADD/RIP-1 pathway mediated by caspase-8 and -10 / protein localization to mitochondrion / positive regulation of response to cytokine stimulus / positive regulation of type I interferon-mediated signaling pathway / peroxisomal membrane / TRAF6 mediated IRF7 activation / negative regulation of type I interferon-mediated signaling pathway / positive regulation of NLRP3 inflammasome complex assembly / negative regulation of viral genome replication / type I interferon-mediated signaling pathway / cellular response to exogenous dsRNA / cytoplasmic pattern recognition receptor signaling pathway / positive regulation of interferon-alpha production / antiviral innate immune response / TRAF6 mediated NF-kB activation / positive regulation of type I interferon production / ubiquitin ligase complex / signaling adaptor activity / positive regulation of defense response to virus by host / activation of innate immune response / positive regulation of interferon-beta production / molecular condensate scaffold activity / Negative regulators of DDX58/IFIH1 signaling / positive regulation of interleukin-8 production / ミトコンドリア / DDX58/IFIH1-mediated induction of interferon-alpha/beta / PKR-mediated signaling / positive regulation of protein import into nucleus / positive regulation of interleukin-6 production / SARS-CoV-1 activates/modulates innate immune responses / positive regulation of DNA-binding transcription factor activity / Ovarian tumor domain proteases / positive regulation of tumor necrosis factor production / TRAF3-dependent IRF activation pathway / defense response to virus / positive regulation of canonical NF-kappaB signal transduction / mitochondrial outer membrane / molecular adaptor activity / defense response to bacterium / positive regulation of protein phosphorylation / 自然免疫系 / protein kinase binding / SARS-CoV-2 activates/modulates innate and adaptive immune responses / シグナル伝達 / positive regulation of transcription by RNA polymerase II / ミトコンドリア / identical protein binding 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | 個体NMR / simulated annealing | ||||||
Model details | lowest energy, model1 | ||||||
データ登録者 | He, L. / Bardiaux, B. / Spehr, J. / Luehrs, T. / Ritter, C. | ||||||
引用 | ジャーナル: Proc.Natl.Acad.Sci.USA / 年: 2016 タイトル: Structure determination of helical filaments by solid-state NMR spectroscopy. 著者: He, L. / Bardiaux, B. / Ahmed, M. / Spehr, J. / Konig, R. / Lunsdorf, H. / Rand, U. / Luhrs, T. / Ritter, C. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 2ms7.cif.gz | 9.7 MB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb2ms7.ent.gz | 8.2 MB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 2ms7.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/ms/2ms7 ftp://data.pdbj.org/pub/pdb/validation_reports/ms/2ms7 | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 11817.363 Da / 分子数: 21 / 断片: UNP RESIDUES 1-100 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: MAVS, IPS1, KIAA1271, VISA / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: Q7Z434 |
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-実験情報
-実験
実験 | 手法: 個体NMR | ||||||||||||||||||||||||||||||||||||
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NMR実験 |
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-試料調製
詳細 |
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試料 |
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試料状態 | イオン強度: 0 / pH: 7 / 圧: ambient / 温度: 278 K |
-NMR測定
NMRスペクトロメーター | タイプ: Bruker Avance / 製造業者: Bruker / モデル: AVANCE / 磁場強度: 600 MHz |
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-解析
NMR software |
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精密化 | 手法: simulated annealing / ソフトェア番号: 1 詳細: Simulated annealing with helical symmetry constrained by strict NCS. | ||||||||||||||||||||||||||||||||||||||||||||
代表構造 | 選択基準: lowest energy | ||||||||||||||||||||||||||||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 500 / 登録したコンフォーマーの数: 15 / 代表コンフォーマー: 1 |