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Yorodumi- PDB-2jxv: Solution structure of a let-7 miRNA:lin-41 mRNA complex from C. e... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2jxv | ||||||
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Title | Solution structure of a let-7 miRNA:lin-41 mRNA complex from C. elegans | ||||||
Components | RNA (33-MER) | ||||||
Keywords | RNA / ASYMMETRIC INTERNAL LOOP / stem-loop / GAAA TETRALOOP / GU MISMATCH / RESIDUAL DIPOLAR COUPLINGS | ||||||
Function / homology | RNA / RNA (> 10) Function and homology information | ||||||
Method | SOLUTION NMR / simulated annealing, energy minimization | ||||||
Authors | Cevec, M. / Thibaudeau, C. / Plavec, J. | ||||||
Citation | Journal: Nucleic Acids Res. / Year: 2008 Title: Solution structure of a let-7 miRNA:lin-41 mRNA complex from C. elegans. Authors: Cevec, M. / Thibaudeau, C. / Plavec, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2jxv.cif.gz | 206.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2jxv.ent.gz | 178.9 KB | Display | PDB format |
PDBx/mmJSON format | 2jxv.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jx/2jxv ftp://data.pdbj.org/pub/pdb/validation_reports/jx/2jxv | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: RNA chain | Mass: 10625.362 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: RNA was prepared by in vitro transcription using T7 RNA polymerase and DNA oligonucleotide template. |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions |
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-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing, energy minimization / Software ordinal: 1 Details: 693 NOE-derived distance restraints, 130 torsion angle restraints, 51 residual dipolar coupling restraints, 32 hydrogen bond restraints and 22 planarity restraints. | ||||||||||||||||
NMR constraints | NOE constraints total: 697 / NOE intraresidue total count: 370 / NOE medium range total count: 57 / NOE sequential total count: 270 | ||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy and least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 10 |