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Yorodumi- PDB-2jx0: The paxillin-binding domain (PBD) of G Protein Coupled Receptor (... -
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-Basic information
Entry | Database: PDB / ID: 2jx0 | ||||||
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Title | The paxillin-binding domain (PBD) of G Protein Coupled Receptor (GPCR)-kinase (GRK) interacting protein 1 (GIT1) | ||||||
Components | ARF GTPase-activating protein GIT1 | ||||||
Keywords | CELL ADHESION / SIGNALING PROTEIN / paxillin binding domain homologue / ANK repeat / Cytoplasm / GTPase activation / Metal-binding / Phosphorylation / Zinc / Zinc-finger | ||||||
Function / homology | Function and homology information negative regulation of ARF protein signal transduction / structural constituent of postsynaptic specialization / RAC2 GTPase cycle / Ephrin signaling / RHOU GTPase cycle / RHOV GTPase cycle / RHOQ GTPase cycle / RAC1 GTPase cycle / motor learning / synaptic vesicle recycling ...negative regulation of ARF protein signal transduction / structural constituent of postsynaptic specialization / RAC2 GTPase cycle / Ephrin signaling / RHOU GTPase cycle / RHOV GTPase cycle / RHOQ GTPase cycle / RAC1 GTPase cycle / motor learning / synaptic vesicle recycling / negative regulation of inflammatory response to wounding / inhibitory synapse / intramembranous ossification / immunological synapse formation / positive regulation of microtubule nucleation / dendritic spine development / regulation of G protein-coupled receptor signaling pathway / regulation of ARF protein signal transduction / gamma-tubulin binding / negative regulation of glycolytic process / neurotransmitter receptor localization to postsynaptic specialization membrane / negative regulation of interleukin-1 beta production / regulation of synaptic vesicle exocytosis / positive regulation of receptor catabolic process / mitotic spindle pole / calyx of Held / excitatory synapse / GABA-ergic synapse / neuron development / ephrin receptor signaling pathway / cellular response to epidermal growth factor stimulus / presynaptic modulation of chemical synaptic transmission / GTPase activator activity / protein tyrosine kinase binding / cell redox homeostasis / locomotory behavior / regulation of cytokinesis / brain development / small GTPase binding / lamellipodium / growth cone / scaffold protein binding / postsynapse / protein phosphatase binding / cellular response to lipopolysaccharide / postsynaptic density / endosome / neuron projection / focal adhesion / centrosome / glutamatergic synapse / synapse / dendrite / protein-containing complex binding / identical protein binding / metal ion binding / cytosol Similarity search - Function | ||||||
Biological species | Rattus norvegicus (Norway rat) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Zhang, Z. / Guibao, C.D. / Simmerman, J.A. / Zheng, J. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2008 Title: GIT1 paxillin-binding domain is a four-helix bundle, and it binds to both paxillin LD2 and LD4 motifs. Authors: Zhang, Z.M. / Simmerman, J.A. / Guibao, C.D. / Zheng, J.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2jx0.cif.gz | 799 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2jx0.ent.gz | 668 KB | Display | PDB format |
PDBx/mmJSON format | 2jx0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jx/2jx0 ftp://data.pdbj.org/pub/pdb/validation_reports/jx/2jx0 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 15029.244 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: Git1 / Plasmid: pET28 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q9Z272 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.2 mM [U-99% 13C; U-99% 15N] GT, 20 mM potassium phosphate, 5 mM DTT, 5 mM EDTA, 95% H2O/5% D2O Solvent system: 95% H2O/5% D2O | ||||||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 20 / pH: 6.50 / Pressure: AMBIENT / Temperature: 310 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software | Name: CYANA / Version: 2 / Developer: GUNTERT, P. ET AL. / Classification: refinement |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 |
NMR representative | Selection criteria: closest to the average |
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 20 |