+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 2jhb | |||||||||
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タイトル | CORE BINDING FACTOR BETA | |||||||||
要素 | PROTEIN (CORE BINDING FACTOR BETA) | |||||||||
キーワード | GENE REGULATION (遺伝子発現の調節) / CORE BINDING FACTOR / TRANSCRIPTION FACTOR (転写因子) | |||||||||
機能・相同性 | 機能・相同性情報 RUNX3 regulates p14-ARF / Transcriptional regulation by RUNX2 / RUNX2 regulates bone development / RUNX1 regulates estrogen receptor mediated transcription / RUNX1 regulates transcription of genes involved in BCR signaling / RUNX1 regulates transcription of genes involved in interleukin signaling / Regulation of RUNX1 Expression and Activity / RUNX1 and FOXP3 control the development of regulatory T lymphocytes (Tregs) / RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known / core-binding factor complex ...RUNX3 regulates p14-ARF / Transcriptional regulation by RUNX2 / RUNX2 regulates bone development / RUNX1 regulates estrogen receptor mediated transcription / RUNX1 regulates transcription of genes involved in BCR signaling / RUNX1 regulates transcription of genes involved in interleukin signaling / Regulation of RUNX1 Expression and Activity / RUNX1 and FOXP3 control the development of regulatory T lymphocytes (Tregs) / RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known / core-binding factor complex / RUNX1 regulates transcription of genes involved in differentiation of myeloid cells / Regulation of RUNX3 expression and activity / positive regulation of CD8-positive, alpha-beta T cell differentiation / negative regulation of CD4-positive, alpha-beta T cell differentiation / lymphocyte differentiation / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function / RUNX1 regulates transcription of genes involved in differentiation of HSCs / Estrogen-dependent gene expression / myeloid cell differentiation / definitive hemopoiesis / cell maturation / 骨化 / osteoblast differentiation / protein polyubiquitination / sequence-specific DNA binding / transcription coactivator activity / regulation of transcription by RNA polymerase II / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / protein-containing complex / DNA binding / 核質 / 細胞核 類似検索 - 分子機能 | |||||||||
生物種 | Mus musculus (ハツカネズミ) | |||||||||
手法 | 溶液NMR / torsion angle dynamics | |||||||||
データ登録者 | Huang, X. / Peng, J. / Speck, N.A. / Bushweller, J.H. | |||||||||
引用 | ジャーナル: Nat.Struct.Biol. / 年: 1999 タイトル: Solution structure of core binding factor beta and map of the CBF alpha binding site. 著者: Huang, X. / Peng, J.W. / Speck, N.A. / Bushweller, J.H. #1: ジャーナル: J.Biol.Chem. / 年: 1998 タイトル: Overexpression, purification, and biophysical characterization of the heterodimerization domain of the core-binding factor beta subunit. 著者: Huang, X. / Crute, B.E. / Sun, C. / Tang, Y.Y. / Kelley 3rd, J.J. / Lewis, A.F. / Hartman, K.L. / Laue, T.M. / Speck, N.A. / Bushweller, J.H. #2: ジャーナル: J.Biomol.Nmr / 年: 1998 タイトル: Complete heteronuclear NMR resonance assignments and secondary structure of core binding factor beta (1-141). 著者: Huang, X. / Speck, N.A. / Bushweller, J.H. | |||||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 2jhb.cif.gz | 890.2 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb2jhb.ent.gz | 743.8 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 2jhb.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/jh/2jhb ftp://data.pdbj.org/pub/pdb/validation_reports/jh/2jhb | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 16757.779 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Mus musculus (ハツカネズミ) / 株: AR58 / 遺伝子: CBFB141 / プラスミド: PGRXCBFB141 / 遺伝子 (発現宿主): GRXCBFB141 / 発現宿主: Escherichia coli (大腸菌) / 株 (発現宿主): AR58 / 参照: UniProt: Q08024 |
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-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR実験 |
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NMR実験の詳細 | Text: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 15N/ 13C AND 15N-LABELED RECOMBINANT CBFB(141) |
-試料調製
試料状態 | pH: 6.5 / 圧: 1 atm / 温度: 293 K |
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結晶化 | *PLUS 手法: その他 / 詳細: NMR |
-NMR測定
NMRスペクトロメーター | タイプ: Varian UNITYPLUS / 製造業者: Varian / モデル: UNITYPLUS / 磁場強度: 500 MHz |
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-解析
NMR software |
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精密化 | 手法: torsion angle dynamics / ソフトェア番号: 1 詳細: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE | |||||||||
NMRアンサンブル | コンフォーマー選択の基準: LEAST RESTRAINT VIOLATION 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 20 |