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Yorodumi- PDB-1ywu: Solution NMR structure of Pseudomonas Aeruginosa protein PA4608. ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1ywu | ||||||
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Title | Solution NMR structure of Pseudomonas Aeruginosa protein PA4608. Northeast Structural Genomics target PaT7 | ||||||
Components | hypothetical protein PA4608Hypothesis | ||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / PA4608 / PaT7 / beta barrel / PilZ domain / PSI / Protein Structure Initiative / Northeast Structural Genomics Consortium / NESG | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Pseudomonas aeruginosa (bacteria) | ||||||
Method | SOLUTION NMR / AutoStructure, simulated annealing, water refinement | ||||||
Authors | Ramelot, T.A. / Yee, A.A. / Cort, J.R. / Semesi, A. / Arrowsmith, C.H. / Kennedy, M.A. / Northeast Structural Genomics Consortium (NESG) | ||||||
Citation | Journal: Proteins / Year: 2007 Title: NMR structure and binding studies confirm that PA4608 from Pseudomonas aeruginosa is a PilZ domain and a c-di-GMP binding protein. Authors: Ramelot, T.A. / Yee, A. / Cort, J.R. / Semesi, A. / Arrowsmith, C.H. / Kennedy, M.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ywu.cif.gz | 790.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ywu.ent.gz | 659 KB | Display | PDB format |
PDBx/mmJSON format | 1ywu.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/yw/1ywu ftp://data.pdbj.org/pub/pdb/validation_reports/yw/1ywu | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 17307.246 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) / Strain: PAO1 / Gene: PA4608 / Plasmid: PET11 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (lamda DE3) / References: UniProt: Q9HVI1 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample conditions | Ionic strength: 250 mM NaCl / pH: 7.1 / Pressure: ambient / Temperature: 293 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||
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Radiation wavelength | Relative weight: 1 | ||||||||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: AutoStructure, simulated annealing, water refinement Software ordinal: 1 Details: The 22 N-terminal residues (MGSSHHHHHHSSGRENLYFQGH) and 2 C-terminal residues (GS) were not included in the structure calculation | ||||||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: all calculated structures submitted Conformers calculated total number: 20 / Conformers submitted total number: 20 |