+Open data
-Basic information
Entry | Database: PDB / ID: 2j2j | ||||||
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Title | Canine adenovirus fibre head at 1.5 A resolution | ||||||
Components | FIBER PROTEINFibrous protein | ||||||
Keywords | VIRAL PROTEIN / FIBER PROTEIN / CANINE ADENOVIRUS / AD / CAR / KNOB / HEAD / FIBER FIBRE / ADENOVIRUS / COXSACKIEVIRUS / ADENOVIRUS RECEPTOR | ||||||
Function / homology | Function and homology information adhesion receptor-mediated virion attachment to host cell / viral capsid / cell adhesion / symbiont entry into host cell / host cell nucleus Similarity search - Function | ||||||
Biological species | CANINE ADENOVIRUS 2 | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.5 Å | ||||||
Authors | Seiradake, E. / Lortat-Jacob, H. / Billet, O. / Kremer, E.J. / Cusack, S. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2006 Title: Structural and Mutational Analysis of Human Ad37 and Canine Adenovirus 2 Fiber Heads in Complex with the D1 Domain of Coxsackie and Adenovirus Receptor. Authors: Seiradake, E. / Lortat-Jacob, H. / Billet, O. / Kremer, E.J. / Cusack, S. | ||||||
History |
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Remark 700 | SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN ... SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2j2j.cif.gz | 467.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2j2j.ent.gz | 382.9 KB | Display | PDB format |
PDBx/mmJSON format | 2j2j.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/j2/2j2j ftp://data.pdbj.org/pub/pdb/validation_reports/j2/2j2j | HTTPS FTP |
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-Related structure data
Related structure data | 2j12C 2j1kC 1qhvS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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-Components
#1: Protein | Mass: 21685.541 Da / Num. of mol.: 6 / Fragment: FIBRE HEAD DOMAIN, RESIDUES 358-542 Source method: isolated from a genetically manipulated source Source: (gene. exp.) CANINE ADENOVIRUS 2 / Plasmid: PQE / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): M15 / References: UniProt: Q65914 #2: Water | ChemComp-HOH / | Sequence details | CONTAINS ONLY FIBRE HEAD DOMAIN, AND N-TERMINAL 6XHIS TAG | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.2 Å3/Da / Density % sol: 44 % |
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Crystal grow | Details: 0.15 M KBR, 30% PEG MONOMETHYL-ETHER 2000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-1 / Wavelength: 0.934 |
Detector | Type: ADSC CCD / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→28.4 Å / Num. obs: 156532 / % possible obs: 95.1 % / Observed criterion σ(I): 0 / Redundancy: 4.1 % / Biso Wilson estimate: 8.1 Å2 / Rmerge(I) obs: 0.1 / Net I/σ(I): 15.3 |
Reflection shell | Resolution: 1.5→1.54 Å / Redundancy: 3.8 % / Rmerge(I) obs: 0.15 / Mean I/σ(I) obs: 7.8 / % possible all: 89.4 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1QHV Resolution: 1.5→106 Å / Cor.coef. Fo:Fc: 0.946 / Cor.coef. Fo:Fc free: 0.918 / SU B: 2.675 / SU ML: 0.047 / Cross valid method: THROUGHOUT / ESU R: 0.101 / ESU R Free: 0.081 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 6.01 Å2
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Refinement step | Cycle: LAST / Resolution: 1.5→106 Å
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Refine LS restraints |
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