+Open data
-Basic information
Entry | Database: PDB / ID: 4n27 | ||||||
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Title | X-ray structure of Brucella abortus RicA | ||||||
Components | Bacterial transferase hexapeptide repeat | ||||||
Keywords | TRANSFERASE / gamma carbonic anhydrase / Zinc binding | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Brucella Abortus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.73 Å | ||||||
Authors | Herrou, J. / Crosson, S. | ||||||
Citation | Journal: Biochemistry / Year: 2013 Title: Molecular Structure of the Brucella abortus Metalloprotein RicA, a Rab2-Binding Virulence Effector. Authors: Herrou, J. / Crosson, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4n27.cif.gz | 211.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4n27.ent.gz | 168.8 KB | Display | PDB format |
PDBx/mmJSON format | 4n27.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/n2/4n27 ftp://data.pdbj.org/pub/pdb/validation_reports/n2/4n27 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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Unit cell |
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-Components
#1: Protein | Mass: 20784.682 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Brucella Abortus (bacteria) / Strain: 2308 / Gene: BAB1_1279, BruAb1_1263 / Plasmid: pET28c / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta (DE3) pLysS / References: UniProt: Q2YQG1 #2: Chemical | ChemComp-ZN / #3: Chemical | ChemComp-PE5 / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.01 Å3/Da / Density % sol: 59.12 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, hanging drop / pH: 4.6 Details: 100 mM Sodium Acetate, pH 4.6, 25 % (w/v) PEG 1000, 200 mM NaCl, 30 mM Ammonium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 21-ID-F / Wavelength: 0.97856 |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Mar 28, 2013 |
Radiation | Monochromator: Diamond [111] / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97856 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→63.77 Å / Num. obs: 39871 / % possible obs: 100 % / Observed criterion σ(F): 3 / Observed criterion σ(I): 1 |
Reflection shell | Resolution: 2.7→2.7983 Å / % possible all: 99.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.73→63.77 Å / SU ML: 0.3 / σ(F): 1.34 / Phase error: 28.15 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.73→63.77 Å
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Refine LS restraints |
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LS refinement shell |
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