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Open data
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Basic information
Entry | Database: PDB / ID: 2bv1 | ||||||
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Title | Regulator of G-protein Signalling 1 (Human) | ||||||
![]() | REGULATOR OF G-PROTEIN SIGNALLING 1 | ||||||
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Function / homology | ![]() leukotriene signaling pathway / G-protein alpha-subunit binding / negative regulation of signal transduction / adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Elkins, J.M. / Yang, X. / Soundararajan, M. / Schoch, G.A. / Haroniti, A. / Sundstrom, M. / Edwards, A. / Arrowsmith, C. / Doyle, D.A. | ||||||
![]() | ![]() Title: Structural diversity in the RGS domain and its interaction with heterotrimeric G protein alpha-subunits. Authors: Soundararajan, M. / Willard, F.S. / Kimple, A.J. / Turnbull, A.P. / Ball, L.J. / Schoch, G.A. / Gileadi, C. / Fedorov, O.Y. / Dowler, E.F. / Higman, V.A. / Hutsell, S.Q. / Sundstrom, M. / ...Authors: Soundararajan, M. / Willard, F.S. / Kimple, A.J. / Turnbull, A.P. / Ball, L.J. / Schoch, G.A. / Gileadi, C. / Fedorov, O.Y. / Dowler, E.F. / Higman, V.A. / Hutsell, S.Q. / Sundstrom, M. / Doyle, D.A. / Siderovski, D.P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 68.9 KB | Display | ![]() |
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PDB format | ![]() | 50.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1zv4C ![]() 2a72C ![]() 2af0C ![]() 2bt2C ![]() 2es0C ![]() 2gtpC ![]() 2i59C ![]() 2ihbC ![]() 2ihdC ![]() 2ik8C ![]() 2jm5C ![]() 2jnuC ![]() 2odeC ![]() 2owiC ![]() 1agrS ![]() 1fqjS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
NCS oper: (Code: given Matrix: (-0.44716, -0.89413, -0.02396), Vector ![]() |
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Components
#1: Protein | Mass: 16575.793 Da / Num. of mol.: 2 / Fragment: RESIDUES 50-192 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Description: THE MAMMALIAN GENE COLLECTION, I.M.A.G.E. CONSORTIUM CLONEID 3916789 Plasmid: PLIC-SGC / Production host: ![]() ![]() ![]() #2: Water | ChemComp-HOH / | ![]() Compound details | INHIBITS SIGNAL TRANSDUCTI | Sequence details | RESIDUES 48-49 OF CHAINS A, B ARE CLONING ARTEFACT | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.8 Å3/Da / Density % sol: 56 % |
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Crystal grow![]() | pH: 8 Details: 4.1M SODIUM FORMATE, 3% GLYCEROL. 1:1 MIXTURE WITH RGS1 PROTEIN AT 23MG/ML., pH 8.00 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS HTC / Detector: IMAGE PLATE / Date: Apr 26, 2005 / Details: OSMIC MIRRORS |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2→32.03 Å / Num. obs: 22497 / % possible obs: 90.6 % / Observed criterion σ(I): 0 / Redundancy: 3.1 % / Rmerge(I) obs: 0.05 / Net I/σ(I): 16.3 |
Reflection shell | Resolution: 2→2.11 Å / Redundancy: 2.9 % / Rmerge(I) obs: 0.34 / Mean I/σ(I) obs: 2.7 / % possible all: 65.7 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRIES 1AGR AND 1FQJ Resolution: 2→57.45 Å / Cor.coef. Fo:Fc: 0.958 / Cor.coef. Fo:Fc free: 0.931 / SU B: 7.626 / SU ML: 0.109 / TLS residual ADP flag: LIKELY RESIDUAL / Cross valid method: THROUGHOUT / ESU R: 0.17 / ESU R Free: 0.165 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 36.81 Å2
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Refinement step | Cycle: LAST / Resolution: 2→57.45 Å
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Refine LS restraints |
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