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- PDB-2b7x: Sequential reorganization of beta-sheet topology by insertion of ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2b7x | ||||||
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Title | Sequential reorganization of beta-sheet topology by insertion of a single strand | ||||||
![]() | Lysozyme![]() | ||||||
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Function / homology | ![]() viral release from host cell by cytolysis / peptidoglycan catabolic process / cell wall macromolecule catabolic process / ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Sagermann, M. / Matthews, B.W. | ||||||
![]() | ![]() Title: Sequential reorganization of beta-sheet topology by insertion of a single strand. Authors: Sagermann, M. / Baase, W.A. / Matthews, B.W. #1: ![]() Title: Long distance conformation changes in a protein engineered by modulated sequence duplication Authors: Sagermann, M. / Gay, L. / Mattews, B.W. #2: ![]() Title: Structural characterization of an engineered tandem repeat contrasts the importance of context and sequence in protein folding Authors: Sagermann, M. / Baase, W.A. / Matthews, B.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 135.9 KB | Display | ![]() |
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PDB format | ![]() | 106.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 3jr6C ![]() 3lzmS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 19233.061 Da / Num. of mol.: 4 Mutation: C54T, C97A, residues (YTIGIG) inserted after residue G30 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-SO4 / ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.23 Å3/Da / Density % sol: 44.9 % |
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Crystal grow![]() | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.8 Details: Polyehtylene glycol 4000, 50mM Ammonium sulfate, , pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Nov 6, 1998 / Details: mirrors |
Radiation | Monochromator: mirrors on si-crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 3→20 Å / Num. all: 14371 / Num. obs: 10387 / % possible obs: 72.3 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.065 / Net I/σ(I): 7.4 |
Reflection shell | Resolution: 3→3.25 Å / % possible all: 66.5 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: pdb entry 3LZM, Resolution: 3→20 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber Details: data was collected on very fragile, thin crystal plates resulting in low occupancy of reflections in data set. Several (non-isomorphous data sets were collected to further verify ...Details: data was collected on very fragile, thin crystal plates resulting in low occupancy of reflections in data set. Several (non-isomorphous data sets were collected to further verify interpretation. Refinement was carried out with the programs refmac and CNS.the sequence between residues 32 and 44 (new mutant numbering) were deleted from the model due to poor density. The c-terminal residues N169 and L170 (new mutant numbering) could not be identified in the density and were therefore omitted.
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Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 3→20 Å
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Refine LS restraints |
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