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Yorodumi- PDB-1zs4: Structure of bacteriophage lambda cII protein in complex with DNA -
+Open data
-Basic information
Entry | Database: PDB / ID: 1zs4 | ||||||
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Title | Structure of bacteriophage lambda cII protein in complex with DNA | ||||||
Components |
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Keywords | transcription/DNA / HELIX-TURN-HELIX / TRANSCRIPTION ACTIVATOR / transcription-DNA COMPLEX | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Enterobacteria phage lambda (virus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 1.7 Å | ||||||
Authors | Jain, D. / Kim, Y. / Maxwell, K.L. / Beasley, S. / Gussin, G.N. / Edwards, A.M. / Darst, S.A. | ||||||
Citation | Journal: Mol.Cell / Year: 2005 Title: Crystal Structure of Bacteriophage lambdacII and Its DNA Complex. Authors: Jain, D. / Kim, Y. / Maxwell, K.L. / Beasley, S. / Zhang, R. / Gussin, G.N. / Edwards, A.M. / Darst, S.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1zs4.cif.gz | 110.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1zs4.ent.gz | 82.7 KB | Display | PDB format |
PDBx/mmJSON format | 1zs4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1zs4_validation.pdf.gz | 467.1 KB | Display | wwPDB validaton report |
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Full document | 1zs4_full_validation.pdf.gz | 476.2 KB | Display | |
Data in XML | 1zs4_validation.xml.gz | 20.2 KB | Display | |
Data in CIF | 1zs4_validation.cif.gz | 30.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zs/1zs4 ftp://data.pdbj.org/pub/pdb/validation_reports/zs/1zs4 | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: DNA chain | Mass: 8264.316 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: SYNTHETIC | ||
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#2: DNA chain | Mass: 8318.400 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: SYNTHETIC | ||
#3: Protein | Mass: 9321.896 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Enterobacteria phage lambda (virus) / Genus: Lambda-like viruses / Gene: CII / Plasmid: pET15b / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P03042 #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 46.61 % | ||||||||||||||||
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Crystal grow | Temperature: 295 K / Method: vapor diffusion / pH: 4.8 Details: PEG 550 MME, SODIUM ACETATE, pH 4.8, VAPOR DIFFUSION, temperature 295K | ||||||||||||||||
Components of the solutions |
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-Data collection
Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X9A / Wavelength: 0.98396, 0.97903, 0.97922, 0.96384 | |||||||||||||||
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Detector | Type: MARRESEARCH / Detector: CCD / Date: Aug 24, 2004 | |||||||||||||||
Radiation | Monochromator: Si 111 / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength |
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Reflection | Resolution: 1.7→50 Å / Num. obs: 54170 / % possible obs: 97.3 % / Rsym value: 0.067 / Net I/σ(I): 8.7 |
-Processing
Software |
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Refinement | Method to determine structure: MAD / Resolution: 1.7→50 Å / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 1.7→50 Å
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