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Yorodumi- PDB-1wg7: Solution structure of pleckstrin homology domain from human KIAA1... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1wg7 | ||||||
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Title | Solution structure of pleckstrin homology domain from human KIAA1058 protein | ||||||
Components | Dedicator of cytokinesis protein 9 | ||||||
Keywords | SIGNALING PROTEIN / pleckstrin homology domain / zizimin1 / structural genomics / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information small GTPase-mediated signal transduction / CDC42 GTPase cycle / endomembrane system / RAC1 GTPase cycle / guanyl-nucleotide exchange factor activity / positive regulation of GTPase activity / Factors involved in megakaryocyte development and platelet production / cadherin binding / membrane / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Suetake, T. / Inoue, K. / Hayashi, F. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: to be published Title: Solution structure of pleckstrin homology domain from human KIAA1058 protein Authors: Suetake, T. / Inoue, K. / Hayashi, F. / Yokoyama, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1wg7.cif.gz | 898.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1wg7.ent.gz | 777.5 KB | Display | PDB format |
PDBx/mmJSON format | 1wg7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wg/1wg7 ftp://data.pdbj.org/pub/pdb/validation_reports/wg/1wg7 | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 16706.740 Da / Num. of mol.: 1 / Fragment: PH domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Description: cell free protein synthesis / Gene: Kazusa cDNA hh12146s1 / Plasmid: P030428-80 / References: UniProt: Q9BZ29 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 0.69mM 13C, 15N-labeled protein; 20mM phosphate buffer; 100mM NaCl; 1mM d10-DTT; 0.02% NaN3 Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 100mM NaCl / pH: 6.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 800 MHz |
-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violation, target function Conformers calculated total number: 100 / Conformers submitted total number: 20 |