+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1wfc | ||||||
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タイトル | STRUCTURE OF APO, UNPHOSPHORYLATED, P38 MITOGEN ACTIVATED PROTEIN KINASE P38 (P38 MAP KINASE) THE MAMMALIAN HOMOLOGUE OF THE YEAST HOG1 PROTEIN | ||||||
要素 | MITOGEN-ACTIVATED PROTEIN KINASE P38 | ||||||
キーワード | TRANSFERASE (転移酵素) / P38 MAP KINASE (P38分裂促進因子活性化タンパク質キナーゼ) / SERINE/THREONINE PROTEIN KINASE | ||||||
機能・相同性 | 機能・相同性情報 positive regulation of cyclase activity / stress-activated protein kinase signaling cascade / Activation of PPARGC1A (PGC-1alpha) by phosphorylation / CD163 mediating an anti-inflammatory response / regulation of synaptic membrane adhesion / stress-induced premature senescence / cell surface receptor protein serine/threonine kinase signaling pathway / 3'-UTR-mediated mRNA stabilization / KSRP (KHSRP) binds and destabilizes mRNA / DSCAM interactions ...positive regulation of cyclase activity / stress-activated protein kinase signaling cascade / Activation of PPARGC1A (PGC-1alpha) by phosphorylation / CD163 mediating an anti-inflammatory response / regulation of synaptic membrane adhesion / stress-induced premature senescence / cell surface receptor protein serine/threonine kinase signaling pathway / 3'-UTR-mediated mRNA stabilization / KSRP (KHSRP) binds and destabilizes mRNA / DSCAM interactions / cartilage condensation / cellular response to UV-B / Platelet sensitization by LDL / mitogen-activated protein kinase p38 binding / positive regulation of myoblast fusion / positive regulation of muscle cell differentiation / negative regulation of hippo signaling / positive regulation of myotube differentiation / NFAT protein binding / Myogenesis / glucose import / Activation of the AP-1 family of transcription factors / ERK/MAPK targets / regulation of cytokine production involved in inflammatory response / p38MAPK cascade / Β酸化 / cellular response to lipoteichoic acid / MAP kinase kinase activity / response to dietary excess / response to muramyl dipeptide / RHO GTPases Activate NADPH Oxidases / MAP kinase activity / regulation of ossification / 分裂促進因子活性化タンパク質キナーゼ / cellular response to vascular endothelial growth factor stimulus / positive regulation of myoblast differentiation / signal transduction in response to DNA damage / chondrocyte differentiation / vascular endothelial growth factor receptor signaling pathway / stress-activated MAPK cascade / skeletal muscle tissue development / lipopolysaccharide-mediated signaling pathway / positive regulation of cardiac muscle cell proliferation / negative regulation of inflammatory response to antigenic stimulus / p38MAPK events / striated muscle cell differentiation / response to muscle stretch / positive regulation of brown fat cell differentiation / positive regulation of interleukin-12 production / osteoclast differentiation / positive regulation of erythrocyte differentiation / DNA damage checkpoint signaling / activated TAK1 mediates p38 MAPK activation / cellular response to ionizing radiation / 細胞分化 / positive regulation of glucose import / placenta development / NOD1/2 Signaling Pathway / response to insulin / 骨 / negative regulation of canonical Wnt signaling pathway / cell morphogenesis / 凝固・線溶系 / cellular response to virus / VEGFA-VEGFR2 Pathway / 紡錘体 / positive regulation of protein import into nucleus / osteoblast differentiation / ADP signalling through P2Y purinoceptor 1 / glucose metabolic process / positive regulation of reactive oxygen species metabolic process / 走化性 / 細胞老化 / cellular response to tumor necrosis factor / peptidyl-serine phosphorylation / protein phosphatase binding / secretory granule lumen / Oxidative Stress Induced Senescence / 血管新生 / Regulation of TP53 Activity through Phosphorylation / cellular response to lipopolysaccharide / ficolin-1-rich granule lumen / transcription by RNA polymerase II / cell surface receptor signaling pathway / intracellular signal transduction / nuclear speck / protein serine kinase activity / protein serine/threonine kinase activity / glutamatergic synapse / apoptotic process / Neutrophil degranulation / positive regulation of gene expression / regulation of transcription by RNA polymerase II / enzyme binding / シグナル伝達 / positive regulation of transcription by RNA polymerase II / ミトコンドリア / extracellular region / 核質 / ATP binding 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | X線回折 / 解像度: 2.3 Å | ||||||
データ登録者 | Wilson, K.P. / Fitzgibbon, M.J. / Caron, P.R. / Griffith, J.P. / Chen, W. / Mccaffrey, P.G. / Chambers, S.P. / Su, M.S.-S. | ||||||
引用 | ジャーナル: J.Biol.Chem. / 年: 1996 タイトル: Crystal structure of p38 mitogen-activated protein kinase. 著者: Wilson, K.P. / Fitzgibbon, M.J. / Caron, P.R. / Griffith, J.P. / Chen, W. / McCaffrey, P.G. / Chambers, S.P. / Su, M.S. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1wfc.cif.gz | 83.5 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1wfc.ent.gz | 63 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1wfc.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/wf/1wfc ftp://data.pdbj.org/pub/pdb/validation_reports/wf/1wfc | HTTPS FTP |
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-関連構造データ
類似構造データ |
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-リンク
-集合体
登録構造単位 |
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単位格子 |
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-要素
#1: タンパク質 | 分子量: 41998.938 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 発現宿主: Escherichia coli (大腸菌) 参照: UniProt: Q16539, 転移酵素; リンを含む基を移すもの; キナーゼ(アルコールにつなげるもの) |
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#2: 水 | ChemComp-HOH / |
-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 1 |
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-試料調製
結晶 | マシュー密度: 2.88 Å3/Da / 溶媒含有率: 57.23 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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結晶化 | pH: 7.5 / 詳細: pH 7.5 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
結晶化 | *PLUS 手法: 蒸気拡散法 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 77 K |
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放射光源 | 波長: 1.5418 |
検出器 | タイプ: RIGAKU / 検出器: IMAGE PLATE / 日付: 1996年5月14日 |
放射 | 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.5418 Å / 相対比: 1 |
反射 | Num. obs: 19927 / % possible obs: 89.6 % / Observed criterion σ(I): 0 / 冗長度: 3.5 % / Rmerge(I) obs: 0.057 |
反射 | *PLUS 最高解像度: 2.3 Å / Num. measured all: 43442 |
反射 シェル | *PLUS 最高解像度: 2.3 Å / 最低解像度: 2.4 Å / % possible obs: 85 % |
-解析
ソフトウェア |
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精密化 | 解像度: 2.3→6 Å / σ(F): 1
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精密化ステップ | サイクル: LAST / 解像度: 2.3→6 Å
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拘束条件 |
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ソフトウェア | *PLUS 名称: X-PLOR / バージョン: 3.1 / 分類: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 | *PLUS
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