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Yorodumi- PDB-1roq: Extending the Family of UNCG-like Tetraloop Motifs: NMR Structure... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1roq | ||||||||||||||||||
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Title | Extending the Family of UNCG-like Tetraloop Motifs: NMR Structure of a CACG Tetraloop from Coxsackievirus B3 | ||||||||||||||||||
Components | 5'-R(*Keywords | RNA / RNA tetra-loop | Function / homology | RNA / RNA (> 10) | Function and homology information Method | SOLUTION NMR / torsion angle dynamics, restrained molecular dynamics | Authors | Du, Z. / Yu, J. / Andino, R. / James, T.L. | Citation | Journal: Biochemistry / Year: 2003 | Title: Extending the Family of UNCG-like Tetraloop Motifs: NMR Structure of a CACG Tetraloop from Coxsackievirus B3 Authors: Du, Z. / Yu, J. / Andino, R. / James, T.L. History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1roq.cif.gz | 89.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1roq.ent.gz | 73 KB | Display | PDB format |
PDBx/mmJSON format | 1roq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ro/1roq ftp://data.pdbj.org/pub/pdb/validation_reports/ro/1roq | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: RNA chain | Mass: 4462.707 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: RNA WAS PREPARED BY IN VITRO TRANSCRIPTION WITH T7 RNA POLYMERASE, this sequence occurs naturally in Coxsackievirus B3 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1-2mM RNA, 25mM sodium phosphate (pH 6.0), 25mM sodium chloride Solvent system: 25mM sodium phosphate (pH 6.0), 25mM sodium chloride | ||||||||||||||||||||
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Sample conditions |
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-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
-Processing
NMR software |
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Refinement | Method: torsion angle dynamics, restrained molecular dynamics Software ordinal: 1 | ||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 20 / Conformers submitted total number: 10 |