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Yorodumi- PDB-1hs2: SOLUTION STRUCTURE OF RNA HAIRPIN LOOP UUAAGU AS PART OF HAIRPIN ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1hs2 | ||||||||||||||||||
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Title | SOLUTION STRUCTURE OF RNA HAIRPIN LOOP UUAAGU AS PART OF HAIRPIN R(GCGUUAAGUCGCA) | ||||||||||||||||||
Components | 5'-R(*Keywords | RNA / ribonucleic acid / hairpin loop / RNA structure / 30S Ribosome | Function / homology | RNA / RNA (> 10) | Function and homology information Method | SOLUTION NMR / restrained molecular dynamics, energy minimization | Authors | Zhang, H. / Fountain, M.A. / Krugh, T.R. | Citation | Journal: Biochemistry / Year: 2001 | Title: Structural characterization of a six-nucleotide RNA hairpin loop found in Escherichia coli, r(UUAAGU). Authors: Zhang, H. / Fountain, M.A. / Krugh, T.R. History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1hs2.cif.gz | 13.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1hs2.ent.gz | 9.7 KB | Display | PDB format |
PDBx/mmJSON format | 1hs2.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hs/1hs2 ftp://data.pdbj.org/pub/pdb/validation_reports/hs/1hs2 | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: RNA chain | Mass: 4157.526 Da / Num. of mol.: 1 / Source method: obtained synthetically |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: 2D NOESY |
-Sample preparation
Details | Contents: 1 mM RNA oligomer - H2O; 3 mM RNA oligomer - D2O |
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Sample conditions | Ionic strength: 0.1 M NaCl, 0.01 M sodium phosphate / pH: 7.0 / Pressure: 1.0 atm / Temperature: 303 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 500 MHz |
-Processing
NMR software |
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Refinement | Method: restrained molecular dynamics, energy minimization / Software ordinal: 1 | ||||||||||||
NMR ensemble | Conformer selection criteria: average structure of 30 conformers Conformers calculated total number: 30 / Conformers submitted total number: 1 |