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Yorodumi- PDB-1r6m: Crystal Structure Of The tRNA Processing Enzyme Rnase pH From Pse... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1r6m | ||||||
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Title | Crystal Structure Of The tRNA Processing Enzyme Rnase pH From Pseudomonas Aeruginosa In Complex With Phosphate | ||||||
Components | Ribonuclease PH | ||||||
Keywords | TRANSFERASE / BETA-ALPHA-BETA-ALPHA FOLD / HEXAMER / PHOSPHATE BOUND | ||||||
Function / homology | Function and homology information tRNA nucleotidyltransferase / tRNA nucleotidyltransferase activity / rRNA catabolic process / tRNA processing / rRNA processing / 3'-5'-RNA exonuclease activity / tRNA binding Similarity search - Function | ||||||
Biological species | Pseudomonas aeruginosa (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / difference Fourier / Resolution: 2 Å | ||||||
Authors | Choi, J.M. / Park, E.Y. / Kim, J.H. / Chang, S.K. / Cho, Y. | ||||||
Citation | Journal: J.BIOL.CHEM. / Year: 2004 Title: Probing the functional importance of the hexameric ring structure of RNase PH Authors: Choi, J.M. / Park, E.Y. / Kim, J.H. / Chang, S.K. / Cho, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1r6m.cif.gz | 61.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1r6m.ent.gz | 45.2 KB | Display | PDB format |
PDBx/mmJSON format | 1r6m.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/r6/1r6m ftp://data.pdbj.org/pub/pdb/validation_reports/r6/1r6m | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | The biological assembly is a hexamer generated from the monomer in the asymmetric unit by the operations; ( -y, x-y, z ), (-x+y, -x, z), (y, x, -z), (x-y, -y , -z) and (-x, -x+y, -z) |
-Components
#1: Protein | Mass: 25683.217 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) / Gene: RPH / Plasmid: pQE30 / Production host: Escherichia coli (E. coli) / Strain (production host): SG13009 / References: UniProt: P50597, tRNA nucleotidyltransferase |
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#2: Chemical | ChemComp-PO4 / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.45 Å3/Da / Density % sol: 49.48 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 5.5 Details: Ammonium phosphate, citrate, sodium chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 ℃ / pH: 8 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 103 K |
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Diffraction source | Source: SYNCHROTRON / Site: PAL/PLS / Beamline: 6B / Wavelength: 1.1272 Å |
Detector | Type: MACSCIENCE / Detector: IMAGE PLATE / Date: May 28, 2003 |
Radiation | Monochromator: Double Crystal Monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1272 Å / Relative weight: 1 |
Reflection | Resolution: 2→30 Å / Num. all: 120641 / Num. obs: 18518 / % possible obs: 94.6 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 |
Reflection shell | Resolution: 2→2.07 Å / % possible all: 89 |
Reflection | *PLUS Num. measured all: 120641 / Rmerge(I) obs: 0.039 |
Reflection shell | *PLUS % possible obs: 89 % / Rmerge(I) obs: 0.332 |
-Processing
Software |
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Refinement | Method to determine structure: difference Fourier / Resolution: 2→24.59 Å / Rfactor Rfree error: 0.009 / Data cutoff high absF: 755476.44 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 1 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 66.0311 Å2 / ksol: 0.366637 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 37.9 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2→24.59 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.13 Å / Rfactor Rfree error: 0.026 / Total num. of bins used: 6
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Xplor file |
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Refinement | *PLUS Highest resolution: 2 Å / Lowest resolution: 30 Å / Rfactor Rfree: 0.279 / Rfactor Rwork: 0.227 / % reflection Rfree: 5 % | ||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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