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Yorodumi- PDB-1qwt: Auto-inhibitory interferon regulation factor-3 (IRF3) transactiva... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1qwt | ||||||
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Title | Auto-inhibitory interferon regulation factor-3 (IRF3) transactivation domain | ||||||
Components | Interferon regulatory factor 3IRF3 | ||||||
Keywords | DNA BINDING PROTEIN | ||||||
Function / homology | Function and homology information IRF3 mediated activation of type 1 IFN / MDA-5 signaling pathway / macrophage apoptotic process / programmed necrotic cell death / TRIF-dependent toll-like receptor signaling pathway / LRR FLII-interacting protein 1 (LRRFIP1) activates type I IFN production / IRF3-mediated induction of type I IFN / positive regulation of type I interferon-mediated signaling pathway / mRNA transcription / toll-like receptor 4 signaling pathway ...IRF3 mediated activation of type 1 IFN / MDA-5 signaling pathway / macrophage apoptotic process / programmed necrotic cell death / TRIF-dependent toll-like receptor signaling pathway / LRR FLII-interacting protein 1 (LRRFIP1) activates type I IFN production / IRF3-mediated induction of type I IFN / positive regulation of type I interferon-mediated signaling pathway / mRNA transcription / toll-like receptor 4 signaling pathway / TRAF6 mediated IRF7 activation / DNA-binding transcription activator activity / immune system process / type I interferon-mediated signaling pathway / cellular response to exogenous dsRNA / cytoplasmic pattern recognition receptor signaling pathway / positive regulation of interferon-alpha production / antiviral innate immune response / positive regulation of type I interferon production / lipopolysaccharide-mediated signaling pathway / TICAM1-dependent activation of IRF3/IRF7 / Regulation of innate immune responses to cytosolic DNA / positive regulation of interferon-beta production / Activation of IRF3, IRF7 mediated by TBK1, IKKε (IKBKE) / Negative regulators of DDX58/IFIH1 signaling / promoter-specific chromatin binding / DDX58/IFIH1-mediated induction of interferon-alpha/beta / ISG15 antiviral mechanism / cellular response to virus / DNA-binding transcription repressor activity, RNA polymerase II-specific / SARS-CoV-1 activates/modulates innate immune responses / Interferon gamma signaling / Interferon alpha/beta signaling / sequence-specific double-stranded DNA binding / TRAF3-dependent IRF activation pathway / regulation of inflammatory response / DNA-binding transcription activator activity, RNA polymerase II-specific / regulation of apoptotic process / defense response to virus / positive regulation of canonical NF-kappaB signal transduction / sequence-specific DNA binding / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / protein domain specific binding / apoptotic process / DNA damage response / chromatin / regulation of transcription by RNA polymerase II / SARS-CoV-2 activates/modulates innate and adaptive immune responses / protein homodimerization activity / positive regulation of transcription by RNA polymerase II / mitochondrion / DNA binding / nucleoplasm / identical protein binding / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MIR / Resolution: 2.1 Å | ||||||
Authors | Qin, B.Y. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 2003 Title: Crystal structure of IRF-3 reveals mechanism of autoinhibition and virus induced phospho-activation Authors: Qin, B.Y. / Liu, C. / Lam, S.S. / Srinath, H. / Delston, R. / Correia, J.J. / Derynck, R. / Lin, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1qwt.cif.gz | 113 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1qwt.ent.gz | 92.4 KB | Display | PDB format |
PDBx/mmJSON format | 1qwt.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/qw/1qwt ftp://data.pdbj.org/pub/pdb/validation_reports/qw/1qwt | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 28159.799 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: IRF3 / Plasmid: pGEX-6P-1 / Production host: Escherichia coli (E. coli) / References: UniProt: Q14653 #2: Chemical | ChemComp-PO4 / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.79 Å3/Da / Density % sol: 55.94 % | ||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 6 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 5.0.1 |
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Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Num. all: 38289 / Num. obs: 36240 / Observed criterion σ(F): 0 |
Reflection | *PLUS Highest resolution: 2.1 Å / Lowest resolution: 100 Å / Num. obs: 38291 / % possible obs: 98.8 % / Num. measured all: 244162 / Rmerge(I) obs: 0.091 |
Reflection shell | *PLUS Highest resolution: 2.1 Å / Lowest resolution: 2.14 Å / % possible obs: 92.2 % / Num. unique obs: 1737 / Rmerge(I) obs: 0.512 / Mean I/σ(I) obs: 2.35 |
-Processing
Software | Name: CNS / Classification: refinement | ||||||||||||||||
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Refinement | Method to determine structure: MIR / Resolution: 2.1→100 Å / σ(F): 0
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Solvent computation | Bsol: 64.995 Å2 / ksol: 0.342877 e/Å3 | ||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.1→100 Å
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Refine LS restraints |
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Refinement | *PLUS % reflection Rfree: 5 % | ||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||
Refine LS restraints | *PLUS
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