+Open data
-Basic information
Entry | Database: PDB / ID: 1q1o | ||||||
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Title | Solution Structure of the PB1 Domain of Cdc24p (Long Form) | ||||||
Components | Cell division control protein 24 | ||||||
Keywords | SIGNALING PROTEIN / PB1 domain / PCCR / PC motif / OPCA motif / yeast / cell polarity / protein-protein interaction | ||||||
Function / homology | Function and homology information regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion / chemotropism / Cdc24p-Far1p-Gbetagamma complex / septin ring organization / protein localization to cell cortex / division septum / PAR polarity complex / cellular bud site selection / incipient cellular bud site / cellular bud tip ...regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion / chemotropism / Cdc24p-Far1p-Gbetagamma complex / septin ring organization / protein localization to cell cortex / division septum / PAR polarity complex / cellular bud site selection / incipient cellular bud site / cellular bud tip / regulation of exit from mitosis / cellular bud neck / mating projection tip / regulation of Rho protein signal transduction / establishment of cell polarity / guanyl-nucleotide exchange factor activity / cell cortex / intracellular signal transduction / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Yoshinaga, S. / Kohjima, M. / Ogura, K. / Yokochi, M. / Takeya, R. / Ito, T. / Sumimoto, H. / Inagaki, F. | ||||||
Citation | Journal: EMBO J. / Year: 2003 Title: The PB1 domain and the PC motif-containing region are structurally similar protein binding modules Authors: Yoshinaga, S. / Kohjima, M. / Ogura, K. / Yokochi, M. / Takeya, R. / Ito, T. / Sumimoto, H. / Inagaki, F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1q1o.cif.gz | 603.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1q1o.ent.gz | 504 KB | Display | PDB format |
PDBx/mmJSON format | 1q1o.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/q1/1q1o ftp://data.pdbj.org/pub/pdb/validation_reports/q1/1q1o | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11211.426 Da / Num. of mol.: 1 / Fragment: PB1 Domain (Long Form) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: Cdc24p / Plasmid: pGEX6p-1 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P11433 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample conditions | Ionic strength: 50mM potassium phosphate buffer; 150mM sodium chloride pH: 6.3 / Pressure: ambient / Temperature: 298 K | |||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |