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Yorodumi- PDB-1pe3: Solution structure of the disulphide-linked dimer of human intest... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1pe3 | ||||||
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Title | Solution structure of the disulphide-linked dimer of human intestinal trefoil factor (TFF3) | ||||||
Components | Trefoil factor 3 | ||||||
Keywords | CELL CYCLE / intestinal trefoil factor / ITF / trefoil factor family / TFF3 / TFF3 dimer / trefoil motif / solution structure / NMR spectroscopy | ||||||
Function / homology | Function and homology information maintenance of gastrointestinal epithelium / regulation of glucose metabolic process / secretory granule / Estrogen-dependent gene expression / extracellular space / extracellular region / identical protein binding Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Muskett, F.W. / May, F.E. / Westley, B.R. / Feeney, J. | ||||||
Citation | Journal: Biochemistry / Year: 2003 Title: Solution structure of the disulfide-linked dimer of human intestinal trefoil factor (TFF3): the intermolecular orientation and interactions are markedly different from those of other dimeric trefoil proteins. Authors: Muskett, F.W. / May, F.E. / Westley, B.R. / Feeney, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1pe3.cif.gz | 1.6 MB | Display | PDBx/mmCIF format |
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PDB format | pdb1pe3.ent.gz | 1.4 MB | Display | PDB format |
PDBx/mmJSON format | 1pe3.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pe/1pe3 ftp://data.pdbj.org/pub/pdb/validation_reports/pe/1pe3 | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 6587.457 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: TFF3 OR ITF OR TFI / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: Q07654 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 2.0mM TFF3 U-15N; 50mM phosphate buffer ph 6.8; 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 100mM / pH: 6.8 / Pressure: ambient / Temperature: 308 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
-Processing
NMR software | Name: DYANA / Version: 1.5 / Developer: Guntert / Classification: refinement |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 |
NMR representative | Selection criteria: closest to the average |
NMR ensemble | Conformer selection criteria: structures with acceptable covalent geometry, structures with favorable non-bond energy, structures with the least restraint violations, structures with the lowest energy, target function Conformers calculated total number: 100 / Conformers submitted total number: 47 |