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Open data
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Basic information
Entry | Database: PDB / ID: 1ois | ||||||
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Title | YEAST DNA TOPOISOMERASE I, N-TERMINAL FRAGMENT | ||||||
![]() | DNA TOPOISOMERASE I![]() | ||||||
![]() | ![]() ![]() ![]() ![]() | ||||||
Function / homology | ![]() chromatin organization => GO:0006325 / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Lue, N. / Sharma, A. / Mondragon, A. / Wang, J.C. | ||||||
![]() | ![]() Title: A 26 kDa yeast DNA topoisomerase I fragment: crystallographic structure and mechanistic implications. Authors: Lue, N. / Sharma, A. / Mondragon, A. / Wang, J.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 69.9 KB | Display | ![]() |
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PDB format | ![]() | 51.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 26085.756 Da / Num. of mol.: 1 / Fragment: N-TERMINAL FRAGMENT, RESIDUES 141 - 363 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Strain: BCY123/PYT-N / Description: YEAST GALACTOSE INDUCIBLE EXPRESSION SYSTEM / Gene: TOP1 / Plasmid: PYT-N / Gene (production host): TOP1 / Production host: ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.8 Å3/Da / Density % sol: 52 % | |||||||||||||||
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Crystal grow![]() | *PLUS Temperature: 18 ℃ / pH: 8 / Method: vapor diffusion | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: ![]() ![]() ![]() |
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Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jul 1, 1994 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Num. obs: 24425 / % possible obs: 95.1 % / Redundancy: 5.9 % / Rmerge(I) obs: 0.061 |
Reflection | *PLUS Highest resolution: 1.85 Å / Lowest resolution: 39 Å / Num. measured all: 127777 |
Reflection shell | *PLUS % possible obs: 80.1 % / Rmerge(I) obs: 0.278 |
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Processing
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Refinement | Resolution: 1.9→8 Å / σ(F): 2
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Displacement parameters | Biso mean: 27 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→8 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_deg / Dev ideal: 1.73 |