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- PDB-1mak: SOLUTION STRUCTURE OF AN ISOLATED ANTIBODY VL DOMAIN -

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Basic information

Entry
Database: PDB / ID: 1mak
TitleSOLUTION STRUCTURE OF AN ISOLATED ANTIBODY VL DOMAIN
ComponentsIGG2A-KAPPA 26-10 FV (LIGHT CHAIN)
KeywordsIMMUNOGLOBULIN
Function / homology
Function and homology information


immunoglobulin complex / antigen binding / adaptive immune response / immune response / extracellular space
Similarity search - Function
Immunoglobulin V-Type / Immunoglobulin V-set domain / Immunoglobulin V-set domain / Immunoglobulin subtype / Immunoglobulin / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulins / Immunoglobulin-like fold ...Immunoglobulin V-Type / Immunoglobulin V-set domain / Immunoglobulin V-set domain / Immunoglobulin subtype / Immunoglobulin / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulins / Immunoglobulin-like fold / Immunoglobulin-like / Sandwich / Mainly Beta
Similarity search - Domain/homology
Ig kappa chain V-II region 26-10
Similarity search - Component
Biological speciesMus musculus (house mouse)
MethodSOLUTION NMR
AuthorsConstantine, K.L. / Friedrichs, M.S. / Metzler, W.J. / Wittekind, M. / Hensley, P. / Mueller, L.
Citation
Journal: J.Mol.Biol. / Year: 1994
Title: Solution structure of an isolated antibody VL domain.
Authors: Constantine, K.L. / Friedrichs, M.S. / Metzler, W.J. / Wittekind, M. / Hensley, P. / Mueller, L.
#1: Journal: To be Published
Title: 26-10 Fab: Digoxin Complex-Affinity and Specificity from Surface Complementarity
Authors: Jeffrey, P.D. / Strong, R.K. / Sieker, L.C. / Chang, C.Y. / Campbell, R.L. / Petsko, G.A. / Haber, E. / Margolies, M.N. / Sheriff, S.
#2: Journal: J.Biomol.NMR / Year: 1993
Title: Aliphatic 1H and 13C Resonance Assignments for the 26-10 Antibody Vl Domain Derived from Heteronuclear Multidimensional NMR Spectroscopy
Authors: Constantine, K.L. / Goldfarb, V. / Wittekind, M. / Friedrichs, M.S. / Anthony, J. / Ng, S.-C. / Mueller, L.
#3: Journal: Proteins / Year: 1993
Title: Characterization of the Backbone Dynamics of an Anti-Digoxin Antibody Vl Domain by Inverse Detected 1H-15N NMR: Comparisons with X-Ray Data for the Fab
Authors: Constantine, K.L. / Friedrichs, M.S. / Goldfarb, V. / Jeffrey, P.D. / Sheriff, S. / Mueller, L.
#4: Journal: J.Mol.Biol. / Year: 1993
Title: Production and Characterization of an Antibody Fv Fragment 15N-Labelled in the Vl Domain Only
Authors: Goldfarb, V. / Wittekind, M. / Jeffrey, P.D. / Mueller, L. / Constantine, K.L.
#5: Journal: Biochemistry / Year: 1992
Title: Sequential 1H and 15N NMR Assignments and Secondary Structure of a Recombinant Anti-Digoxin Antibody Vl Domain
Authors: Constantine, K.L. / Goldfarb, V. / Wittekind, M. / Anthony, J. / Ng, S.-C. / Mueller, L.
History
DepositionSep 16, 1993Processing site: BNL
Revision 1.0Jan 31, 1994Provider: repository / Type: Initial release
Revision 1.1Mar 3, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Nov 29, 2017Group: Derived calculations / Other
Category: pdbx_database_status / pdbx_struct_assembly ...pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list / struct_conf
Item: _pdbx_database_status.process_site

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: IGG2A-KAPPA 26-10 FV (LIGHT CHAIN)


Theoretical massNumber of molelcules
Total (without water)12,2861
Polymers12,2861
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_5551
Atom site foot note1: CIS PROLINE - PRO 8 MODEL 1 / 2: CIS PROLINE - PRO 100 MODEL 1 / 3: CIS PROLINE - PRO 8 MODEL 2 / 4: CIS PROLINE - PRO 100 MODEL 2 / 5: CIS PROLINE - PRO 8 MODEL 3 / 6: CIS PROLINE - PRO 100 MODEL 3 / 7: CIS PROLINE - PRO 8 MODEL 4 / 8: CIS PROLINE - PRO 100 MODEL 4 / 9: CIS PROLINE - PRO 8 MODEL 5 / 10: CIS PROLINE - PRO 100 MODEL 5 / 11: CIS PROLINE - PRO 8 MODEL 6 / 12: CIS PROLINE - PRO 100 MODEL 6 / 13: CIS PROLINE - PRO 8 MODEL 7 / 14: CIS PROLINE - PRO 100 MODEL 7 / 15: CIS PROLINE - PRO 8 MODEL 8 / 16: CIS PROLINE - PRO 100 MODEL 8 / 17: CIS PROLINE - PRO 8 MODEL 9 / 18: CIS PROLINE - PRO 100 MODEL 9 / 19: CIS PROLINE - PRO 8 MODEL 10 / 20: CIS PROLINE - PRO 100 MODEL 10 / 21: CIS PROLINE - PRO 8 MODEL 11 / 22: CIS PROLINE - PRO 100 MODEL 11 / 23: CIS PROLINE - PRO 8 MODEL 12 / 24: CIS PROLINE - PRO 100 MODEL 12 / 25: CIS PROLINE - PRO 8 MODEL 13 / 26: CIS PROLINE - PRO 100 MODEL 13 / 27: CIS PROLINE - PRO 8 MODEL 14 / 28: CIS PROLINE - PRO 100 MODEL 14 / 29: CIS PROLINE - PRO 8 MODEL 15 / 30: CIS PROLINE - PRO 100 MODEL 15
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)15 / -
Representative

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Components

#1: Antibody IGG2A-KAPPA 26-10 FV (LIGHT CHAIN)


Mass: 12285.821 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / References: UniProt: P01631

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR

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Processing

Software
NameClassification
X-PLORmodel building
X-PLORrefinement
X-PLORphasing
NMR software
NameDeveloperClassification
DIANAGUNTERT,WUTHRICHrefinement
X-PLORBRUNGERrefinement
NMR ensembleConformers submitted total number: 15

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