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Open data
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Basic information
Entry | Database: PDB / ID: 3w7y | ||||||
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Title | 0.92A structure of 2Zn human insulin at 100K | ||||||
![]() | (Insulin![]() | ||||||
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Function / homology | ![]() negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / regulation of cellular amino acid metabolic process / negative regulation of fatty acid metabolic process / negative regulation of feeding behavior / Signaling by Insulin receptor / nitric oxide-cGMP-mediated signaling / IRS activation / Insulin processing / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Sakabe, N. / Sakabe, K. / Sasaki, K. / Murayoshi, M. | ||||||
![]() | ![]() Title: 0.92A structure of 2Zn human insulin at 100K Authors: Sakabe, N. / Sakabe, K. / Sasaki, K. / Murayoshi, M. #1: ![]() Title: Automatic Weissenberg data collection system for time-resolved protein crystallography Authors: Sakabe, N. / Sakabe, K. / Higashi, T. / Igarashi, N. / Suzuki, M. / Watanabe, N. / Sasaki, K. #2: ![]() Title: Rotated-inclined focusing monochromator with simultaneous tuning of asymmetry factor and radius of curvature over a wide wavelength range Authors: Watanabe, N. / Suzuki, M. / Higashi, Y. / Sakabe, N. #3: ![]() Title: Large-Format Imaging Plate and Weissenberg Camera for Accurate Protein Crystallographic Data Collection Using Synchrotron Radiation Authors: Sakabe, K. / Sasaki, K. / Watanabe, N. / Suzuki, M. / Wang, Z.G. / Miyahara, J. / Sakabe, N. #4: ![]() Title: The structure of 2Zn pig insulin crystals at 1.5A resolution Authors: Baker, E. / Blundell, T.L. / Cutfield, J.F. / Cutfield, S.M. / Dodson, E.J. / Dodson, G. / Hodgkin, D.M.C. / Hubbard, E. / Isaacs, N.W. / Reynolds, C.D. / Sakabe, K. / Sakabe, N. / Vijayan, N.M. #5: ![]() Title: A statistical evaluation of absorption Authors: Katayama, C. / Sakabe, N. / Sakabe, K. #6: ![]() Title: The processing of diffraction data taken on a screenless Weissenberg camera for macromolecular crystallography Authors: Higashi, T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 61.3 KB | Display | ![]() |
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PDB format | ![]() | 49.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Details | The biological assembly is a hexamer generated from the dimer in the asymmetric unit by the operations: -y, x-y, z and -x+y, -x, z. |
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Components
#1: Protein/peptide | ![]() Mass: 2383.698 Da / Num. of mol.: 2 / Fragment: UNP residues 90-110 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #2: Protein/peptide | ![]() Mass: 3433.953 Da / Num. of mol.: 2 / Fragment: UNP residues 25-54 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #3: Chemical | #4: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.85 Å3/Da / Density % sol: 33.39 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.67 Details: 10mg/ml protein, 0.1M Sodium Citrate, 22% DMF(v/v) and 0.08% Zinc chloride(w/v), pH 8.67, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: Fully automatic high speed Weissenberg data collection system called Galaxy Detector: IMAGE PLATE / Date: Dec 23, 2003 Details: The vertically focusing 1m long bent mirror of Pt-coated fused silica is 21m from the SR source point and 7m from the focus point. |
Radiation | Monochromator: Rotated-inclined focusing Si(111) crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 0.7→50 Å / Num. obs: 57006 / % possible obs: 91.73 % / Redundancy: 4.18 % / Rmerge(I) obs: 0.05579 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 14.737 Å2
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Refinement step | Cycle: LAST / Resolution: 0.92→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 0.92→0.944 Å / Total num. of bins used: 20
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