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- PDB-1ib1: CRYSTAL STRUCTURE OF THE 14-3-3 ZETA:SEROTONIN N-ACETYLTRANSFERAS... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1ib1 | ||||||
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Title | CRYSTAL STRUCTURE OF THE 14-3-3 ZETA:SEROTONIN N-ACETYLTRANSFERASE COMPLEX | ||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Obsil, T. / Ghirlando, R. / Klein, D.C. / Ganguly, S. / Dyda, F. | ||||||
![]() | ![]() Title: Crystal structure of the 14-3-3zeta:serotonin N-acetyltransferase complex. a role for scaffolding in enzyme regulation. Authors: Obsil, T. / Ghirlando, R. / Klein, D.C. / Ganguly, S. / Dyda, F. | ||||||
History |
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Remark 999 | SEQUENCE SEROTONIN N-ACETYLTRANSFERASE (CHAINS E,F,G,H) WAS EXPRESSED TRUNCATED (ONLY RESIDUES 1-201). |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 332.4 KB | Display | ![]() |
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PDB format | ![]() | 272.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 27777.092 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() #2: Protein | ![]() Mass: 22272.500 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() References: UniProt: Q29495, ![]() #3: Chemical | ChemComp-COT / #4: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.54 Å3/Da / Density % sol: 51.59 % | ||||||||||||||||||||||||||||||
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Crystal grow![]() | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: PEG 4000, magnesium chloride, lithium sulfate, DTT, EDTA, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 95 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE / Date: Apr 10, 2000 / Details: TOTAL REFLECTION MIRRORS |
Radiation | Monochromator: NI FILTER / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.7→20 Å / Num. all: 78001 / Num. obs: 44944 / % possible obs: 84.9 % / Observed criterion σ(I): 0 / Redundancy: 1.74 % / Biso Wilson estimate: 26 Å2 / Rmerge(I) obs: 0.102 / Rsym value: 0.102 / Net I/σ(I): 5.6 |
Reflection shell | Resolution: 2.7→2.78 Å / Redundancy: 1.5 % / Rmerge(I) obs: 0.251 / Mean I/σ(I) obs: 2.09 / Num. unique all: 3188 / Rsym value: 0.251 / % possible all: 72 |
Reflection | *PLUS Num. measured all: 78001 |
Reflection shell | *PLUS % possible obs: 72 % |
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Processing
Software |
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 1A4O AND 1CJW Resolution: 2.7→20 Å / Isotropic thermal model: isotropic / Cross valid method: THROUGHOUT / σ(F): 1 / σ(I): 0.5 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 16.3 Å2 | |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.7→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.7→2.82 Å / Rfactor Rfree error: 0.034
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