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Yorodumi- PDB-1i3p: THE 3.1 ANGSTROM RESOLUTION CRYSTAL STRUCTURE OF A MUTATED BACULO... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1i3p | ||||||
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Title | THE 3.1 ANGSTROM RESOLUTION CRYSTAL STRUCTURE OF A MUTATED BACULOVIRUS P35 AFTER CASPASE CLEAVAGE | ||||||
Components | EARLY 35 KDA PROTEIN | ||||||
Keywords | APOPTOSIS / helix-turn-helix / reactive site loop / hairpin loop | ||||||
Function / homology | Function and homology information symbiont-mediated suppression of host RNAi-mediated antiviral immune response / : / cysteine-type endopeptidase inhibitor activity / cysteine-type endopeptidase inhibitor activity involved in apoptotic process / : / negative regulation of apoptotic process Similarity search - Function | ||||||
Biological species | Autographa californica nucleopolyhedrovirus | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.1 Å | ||||||
Authors | dela Cruz, W.P. / Lemongello, D. / Friesen, P.D. / Fisher, A.J. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2001 Title: Crystal structure of baculovirus P35 reveals a novel conformational change in the reactive site loop after caspase cleavage. Authors: dela Cruz, W.P. / Friesen, P.D. / Fisher, A.J. #1: Journal: Embo J. / Year: 1999 Title: Crystal structure of baculovirus P35: role of a novel reactive site loop in apoptotic inhibition Authors: Fisher, A.J. / dela Cruz, W.P. / Zoog, S.J. / Schneider, C.L. / Friesen, P.D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1i3p.cif.gz | 61.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1i3p.ent.gz | 44.2 KB | Display | PDB format |
PDBx/mmJSON format | 1i3p.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/i3/1i3p ftp://data.pdbj.org/pub/pdb/validation_reports/i3/1i3p | HTTPS FTP |
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-Related structure data
Related structure data | 1i3sC 1p35S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | The biological assembly is a monomer |
-Components
#1: Protein | Mass: 34712.434 Da / Num. of mol.: 1 / Mutation: V71P Source method: isolated from a genetically manipulated source Source: (gene. exp.) Autographa californica nucleopolyhedrovirus Genus: NucleopolyhedrovirusAlphabaculovirus / Gene: P35 / Plasmid: PET22B+ / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: P08160 |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.88 Å3/Da / Density % sol: 57.34 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Method: vapor diffusion, hanging drop / pH: 6 Details: 9-13% PEG 20,000, 100-400 mM NaCl, 100 mM MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal | *PLUS Density % sol: 62.1 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 25 ℃ / pH: 7 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 170 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL9-1 / Wavelength: 0.98 Å |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Dec 6, 1999 / Details: mirrors |
Radiation | Monochromator: Ni MIRROR + Ni FILTER / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 3.1→15 Å / Num. all: 80534 / Num. obs: 7577 / % possible obs: 99 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 1 / Redundancy: 10.63 % / Biso Wilson estimate: 69.8 Å2 / Rmerge(I) obs: 0.054 / Net I/σ(I): 470.1 |
Reflection shell | Resolution: 3.1→3.21 Å / Redundancy: 4.9 % / Rmerge(I) obs: 0.395 / % possible all: 99.6 |
Reflection | *PLUS Num. measured all: 80534 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1P35 Resolution: 3.1→14.95 Å / Rfactor Rfree error: 0.016 / Data cutoff high absF: 934677.25 / Data cutoff low absF: 0 / Isotropic thermal model: GROUP / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 49.08 Å2 / ksol: 0.239 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 75.6 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3.1→14.95 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.1→3.29 Å / Rfactor Rfree error: 0.049 / Total num. of bins used: 6
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Software | *PLUS Name: CNS / Version: 1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS σ(F): 0 / % reflection Rfree: 4.6 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 75.6 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.384 / % reflection Rfree: 4.9 % / Rfactor Rwork: 0.314 |