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- PDB-1gbq: SOLUTION NMR STRUCTURE OF THE GRB2 N-TERMINAL SH3 DOMAIN COMPLEXE... -
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Basic information
Entry | Database: PDB / ID: 1gbq | ||||||
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Title | SOLUTION NMR STRUCTURE OF THE GRB2 N-TERMINAL SH3 DOMAIN COMPLEXED WITH A TEN-RESIDUE PEPTIDE DERIVED FROM SOS DIRECT REFINEMENT AGAINST NOES, J-COUPLINGS, AND 1H AND 13C CHEMICAL SHIFTS, MINIMIZED AVERAGE STRUCTURE | ||||||
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![]() | COMPLEX (SIGNAL TRANSDUCTION/PEPTIDE) / COMPLEX (SIGNAL TRANSDUCTION-PEPTIDE) / ![]() | ||||||
Function / homology | ![]() Signalling to RAS / Activated NTRK2 signals through FRS2 and FRS3 / SHC1 events in ERBB4 signaling / GRB2 events in ERBB2 signaling / EGFR Transactivation by Gastrin / SHC-mediated cascade:FGFR3 / SHC-mediated cascade:FGFR4 / SHC-mediated cascade:FGFR1 / SHC-mediated cascade:FGFR2 / MET activates PI3K/AKT signaling ...Signalling to RAS / Activated NTRK2 signals through FRS2 and FRS3 / SHC1 events in ERBB4 signaling / GRB2 events in ERBB2 signaling / EGFR Transactivation by Gastrin / SHC-mediated cascade:FGFR3 / SHC-mediated cascade:FGFR4 / SHC-mediated cascade:FGFR1 / SHC-mediated cascade:FGFR2 / MET activates PI3K/AKT signaling / PI-3K cascade:FGFR3 / PI-3K cascade:FGFR4 / SHC-related events triggered by IGF1R / Signal regulatory protein family interactions / PI-3K cascade:FGFR1 / PI-3K cascade:FGFR2 / SOS-mediated signalling / FRS-mediated FGFR3 signaling / FRS-mediated FGFR4 signaling / MET activates RAS signaling / MET activates PTPN11 / Costimulation by the CD28 family / FRS-mediated FGFR1 signaling / FRS-mediated FGFR2 signaling / PI3K events in ERBB2 signaling / Tie2 Signaling / GRB2 events in EGFR signaling / SHC1 events in EGFR signaling / Interleukin-15 signaling / FLT3 Signaling / CD28 dependent Vav1 pathway / MET activates RAP1 and RAC1 / RHOU GTPase cycle / Signaling by CSF3 (G-CSF) / MET receptor recycling / NCAM signaling for neurite out-growth / Erythropoietin activates RAS / Regulation of KIT signaling / GAB1 signalosome / Role of LAT2/NTAL/LAB on calcium mobilization / PI3K Cascade / Negative regulation of FGFR3 signaling / Negative regulation of FGFR4 signaling / anatomical structure formation involved in morphogenesis / Downstream signal transduction / Negative regulation of FGFR1 signaling / Negative regulation of FGFR2 signaling / Spry regulation of FGF signaling / Insulin receptor signalling cascade / FCERI mediated MAPK activation / Negative regulation of MET activity / lymphocyte homeostasis / Signal attenuation / RHO GTPases Activate WASPs and WAVEs / NRAGE signals death through JNK / Signaling by SCF-KIT / EGFR downregulation / RAC1 GTPase cycle / GRB2:SOS provides linkage to MAPK signaling for Integrins / guanyl-nucleotide exchange factor adaptor activity / FCERI mediated Ca+2 mobilization / Grb2-EGFR complex / RAF/MAP kinase cascade / Interleukin receptor SHC signaling / G alpha (12/13) signalling events / midbrain morphogenesis / PIP3 activates AKT signaling / regulation of pro-B cell differentiation / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / Regulation of signaling by CBL / ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Wittekind, M. / Mapelli, C. / Lee, V. / Goldfarb, V. / Friedrichs, M.S. / Meyers, C.A. / Mueller, L. | ||||||
![]() | ![]() Title: Solution structure of the Grb2 N-terminal SH3 domain complexed with a ten-residue peptide derived from SOS: direct refinement against NOEs, J-couplings and 1H and 13C chemical shifts. Authors: Wittekind, M. / Mapelli, C. / Lee, V. / Goldfarb, V. / Friedrichs, M.S. / Meyers, C.A. / Mueller, L. #1: ![]() Title: Orientation of Peptide Fragments from SOS Proteins Bound to the N-Terminal SH3 Domain of Grb2 Determined by NMR Spectroscopy Authors: Wittekind, M. / Mapelli, C. / Farmer II, B.T. / Suen, K.L. / Goldfarb, V. / Tsao, J. / Lavoie, T. / Barbacid, M. / Meyers, C.A. / Mueller, L. #2: ![]() Title: Molecular Cloning of the Mouse Grb2 Gene: Differential Interaction of the Grb2 Adaptor Protein with Epidermal Growth Factor and Nerve Growth Factor Receptors Authors: Suen, K.L. / Bustelo, X.R. / Pawson, T. / Barbacid, M. #3: ![]() Title: Identification of Murine Homologues of the Drosophila Son of Sevenless Gene: Potential Activators of Ras Authors: Bowtell, D. / Fu, P. / Simon, M. / Senior, P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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PDBx/mmCIF format | ![]() | 30 KB | Display | ![]() |
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PDB format | ![]() | 22.3 KB | Display | ![]() |
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-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | ![]() Mass: 8454.495 Da / Num. of mol.: 1 / Fragment: N-TERMINAL SH3 DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 1197.478 Da / Num. of mol.: 1 / Fragment: RESIDUES 1135 - 1144, AC-VPPPVPPRRR-NH2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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NMR experiment | Type![]() |
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Sample preparation
Sample conditions | pH: 6.0 / Temperature: 298. K |
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Crystal grow![]() | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian UNITYPLUS / Manufacturer: Varian / Model![]() |
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Processing
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NMR software |
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Refinement | Method: DG, SA / Software ordinal: 1 Details: TWO ENSEMBLES WERE CALCULATED. BOTH USED THE SAME SET OF NOE/ANGLE/J-COUPLING RESTRAINTS, BUT THEY DIFFER IN THAT THE 2ND ENSEMBLE ALSO INCLUDED 1H AND 13C CHEMICAL SHIFTS AS RESTRAINTS (SEE ...Details: TWO ENSEMBLES WERE CALCULATED. BOTH USED THE SAME SET OF NOE/ANGLE/J-COUPLING RESTRAINTS, BUT THEY DIFFER IN THAT THE 2ND ENSEMBLE ALSO INCLUDED 1H AND 13C CHEMICAL SHIFTS AS RESTRAINTS (SEE PRIMARY REFERENCE FOR DETAILS). | |||||||||
NMR ensemble | Conformers calculated total number: 200 / Conformers submitted total number: 1 |